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Literature summary for 1.7.1.2 extracted from

  • Hipkin, C.R.; Ali, A.H.; Cannons, A.
    Structure and properties of assimilatory nitrate reductase from the yeast Candida nitratophila (1986), J. Gen. Microbiol., 132, 1997-2003.
No PubMed abstract available
Search for more literature for 1.7.1.2

General Stability

General Stability Organism
Labile to dilution with protein-free buffer, 2.5 mg bovine serum albumin, stabilizes [Candida] nitratophila

Inhibitors

Inhibitors Comment Organism Structure
cyanide preincubation with NAD(P)H and cyanide: inactivation [Candida] nitratophila
NAD(P)H preincubation with NAD(P)H alone: activation; preincubation with NAD(P)H and cyanide: inactivation [Candida] nitratophila
p-chloromercuribenzoate
-
 [Candida] nitratophila

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
 NADH
-
 [Candida] nitratophila
0.03
-
 NADPH
-
 [Candida] nitratophila
0.11
-
 nitrate cosubstrate NADPH [Candida] nitratophila
0.12
-
 nitrate cofactor NADH [Candida] nitratophila

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
95000
-
 4 * 95000, Candida nitratophila, SDS-PAGE [Candida] nitratophila
365000
-
 Candida nitratophila, sedimentation equilibrium centrifugation [Candida] nitratophila

Organism

Organism UniProt Comment Textmining
[Candida] nitratophila
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 [Candida] nitratophila

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
217.4
-
-
 [Candida] nitratophila

Storage Stability

Storage Stability Organism
4°C, 50% loss of activity after 7 days [Candida] nitratophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NAD(P)H + nitrate
-
 [Candida] nitratophila NAD(P)+ + nitrite + H2O
-
 ?
nitrate + NADH + H+
-
 [Candida] nitratophila nitrite + NAD+ + H2O
-
 r

Subunits

Subunits Comment Organism
tetramer 4 * 95000, Candida nitratophila, SDS-PAGE [Candida] nitratophila

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information above 6000 [Candida] nitratophila

Cofactor

Cofactor Comment Organism Structure
cytochrome b557 contains cytochrome b557 [Candida] nitratophila
FAD maximum activity requires addition of flavin nucleotides, FAD more effective than FMN [Candida] nitratophila
FMN maximum activity requires addition of flavin nucleotides, FAD is more effective than FMN [Candida] nitratophila
NADH preincubation with NAD(P)H and CN- causes inactivation, preincubation with NAD(P)H activates [Candida] nitratophila
NADH utilizes both NADH and NADPH, more active with NADH [Candida] nitratophila
NADPH utilizes both NADH and NADPH, more active with NADH [Candida] nitratophila
NADPH preincubation with NAD(P)H and CN- causes inactivation, preincubation with NAD(P)H alone activates [Candida] nitratophila