Cloned (Comment) | Organism |
---|---|
gene azoR, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) | Rhodococcus opacus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 10% inhibition at 0.05 mM | Rhodococcus opacus | |
Fe2+ | 13% inhibition at 0.05 mM | Rhodococcus opacus | |
Fe3+ | 14% inhibition at 0.05 mM | Rhodococcus opacus | |
Mg2+ | 8% inhibition at 0.05 mM | Rhodococcus opacus | |
additional information | the enzyme is oxygen-insensitive | Rhodococcus opacus | |
Zn2+ | 16% inhibition at 0.05 mM | Rhodococcus opacus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | nonlinear Michaelis-Menten kinetics | Rhodococcus opacus | |
0.0107 | - |
NADH | recombinant enzyme, pH 6.0, 25°C | Rhodococcus opacus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 43% activation at 0.05 mM | Rhodococcus opacus | |
Mn2+ | 119% activation at 0.05 mM | Rhodococcus opacus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-(dimethylamino)azobenzene + NADH + H+ | Rhodococcus opacus | - |
N,N-dimethyl-1,4-phenylenediamine + aniline + NAD+ | - |
? | |
4-(dimethylamino)azobenzene + NADH + H+ | Rhodococcus opacus 1CP | - |
N,N-dimethyl-1,4-phenylenediamine + aniline + NAD+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus opacus | A0A1B1KJ01 | - |
- |
Rhodococcus opacus 1CP | A0A1B1KJ01 | - |
- |
Oxidation Stability | Organism |
---|---|
the enzyme is oxygen-insensitive | Rhodococcus opacus |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Rhodococcus opacus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
141.25 | - |
purified recombinant enzyme, pH 4.0, 25°C | Rhodococcus opacus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-(4-dimethylaminophenylazo) benzoic acid + NADH + H+ | i.e. methyl red | Rhodococcus opacus | N,N'-dimethyl-p-phenylenediamine + 2-aminobenzoic acid + NAD+ | - |
? | |
2-(4-dimethylaminophenylazo) benzoic acid + NADH + H+ | i.e. methyl red | Rhodococcus opacus 1CP | N,N'-dimethyl-p-phenylenediamine + 2-aminobenzoic acid + NAD+ | - |
? | |
4-(dimethylamino)azobenzene + NADH + H+ | - |
Rhodococcus opacus | N,N-dimethyl-1,4-phenylenediamine + aniline + NAD+ | - |
? | |
4-(dimethylamino)azobenzene + NADH + H+ | i.e. methyl yellow | Rhodococcus opacus | N,N-dimethyl-1,4-phenylenediamine + aniline + NAD+ | - |
? | |
4-(dimethylamino)azobenzene + NADH + H+ | - |
Rhodococcus opacus 1CP | N,N-dimethyl-1,4-phenylenediamine + aniline + NAD+ | - |
? | |
4-(dimethylamino)azobenzene + NADH + H+ | i.e. methyl yellow | Rhodococcus opacus 1CP | N,N-dimethyl-1,4-phenylenediamine + aniline + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 25000, recombinant His-tagged enzyme, SDS-PAGE, x * 25350, about, His-tagged enzyme, sequence calculation | Rhodococcus opacus |
More | enzyme structure modeling, overview | Rhodococcus opacus |
Synonyms | Comment | Organism |
---|---|---|
azo-dye reductase | UniProt | Rhodococcus opacus |
AzoR | - |
Rhodococcus opacus |
AzoRo | - |
Rhodococcus opacus |
flavin-containing oxygen-insensitive azoreductase | - |
Rhodococcus opacus |
FMN-dependent NADH-azoreductase | UniProt | Rhodococcus opacus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
53 | - |
- |
Rhodococcus opacus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 50 | the enzyme activity drops largely after 50°C, and there is no activity left when temperature rises to 70°C | Rhodococcus opacus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 40 | purified recombinant enzyme, pH 7.0, 0.1 M sodium phosphate buffer, 30 min, stable at | Rhodococcus opacus |
55 | - |
purified recombinant enzyme, unfolding temperature | Rhodococcus opacus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
- |
Rhodococcus opacus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | a flavin-containing enzyme the enzyme binds 1 FMN per subunit | Rhodococcus opacus | |
additional information | no activity with NADPH | Rhodococcus opacus | |
NADH | dependent on | Rhodococcus opacus |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the azoreductase type 1 family | Rhodococcus opacus |
additional information | enzyme structure modeling, overview | Rhodococcus opacus |
physiological function | the enzyme catalyses the initial degradation step of azo dyes, characterized by one or more R1-N=N-R2 bonds | Rhodococcus opacus |