Cloned (Comment) | Organism |
---|---|
mutant S534A is expressed in Pichia pastoris strainKM71 | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
S534A | the mutant is not inhibited by 14-3-3 proteins | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
14-3-3 protein isoform chi | noncompetitive inhibition with a preferential binding to the substrate-bound state of the enzyme | Arabidopsis thaliana | |
14-3-3 protein isoform epsilon | noncompetitive inhibition with a preferential binding to the substrate-bound state of the enzyme | Arabidopsis thaliana | |
14-3-3 protein isoform kappa | noncompetitive inhibition with a preferential binding to the substrate-bound state of the enzyme | Arabidopsis thaliana | |
14-3-3 protein isoform lambda | noncompetitive inhibition with a preferential binding to the substrate-bound state of the enzyme | Arabidopsis thaliana | |
14-3-3 protein isoform ni | noncompetitive inhibition with a preferential binding to the substrate-bound state of the enzyme | Arabidopsis thaliana | |
14-3-3 protein isoform omega | noncompetitive inhibition with a preferential binding to the substrate-bound state of the enzyme | Arabidopsis thaliana | |
14-3-3 protein isoform phi | noncompetitive inhibition with a preferential binding to the substrate-bound state of the enzyme | Arabidopsis thaliana | |
14-3-3 protein isoform theta | noncompetitive inhibition with a preferential binding to the substrate-bound state of the enzyme | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.197 | - |
nitrate | 22°C, pH not specified in the publication | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrate + NADH + H+ | Arabidopsis thaliana | - |
nitrite + NAD+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | - |
Arabidopsis thaliana |
Purification (Comment) | Organism |
---|---|
SourceQ15 column chromatography and Ni-NTA column chromatography | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrate + NADH + H+ | - |
Arabidopsis thaliana | nitrite + NAD+ + H2O | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
33 | - |
nitrate | 22°C, pH not specified in the publication | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Arabidopsis thaliana | |
NADH | - |
Arabidopsis thaliana |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00006 | - |
14-3-3 protein isoform omega | 22°C, pH not specified in the publication | Arabidopsis thaliana | |
0.00008 | - |
14-3-3 protein isoform lambda | 22°C, pH not specified in the publication | Arabidopsis thaliana | |
0.00014 | - |
14-3-3 protein isoform kappa | 22°C, pH not specified in the publication | Arabidopsis thaliana | |
0.00023 | - |
14-3-3 protein isoform ni | 22°C, pH not specified in the publication | Arabidopsis thaliana | |
0.00031 | - |
14-3-3 protein isoform phi | 22°C, pH not specified in the publication | Arabidopsis thaliana | |
0.00034 | - |
14-3-3 protein isoform chi | 22°C, pH not specified in the publication | Arabidopsis thaliana | |
0.00076 | - |
14-3-3 protein isoform theta | 22°C, pH not specified in the publication | Arabidopsis thaliana |