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Literature summary for 1.7.1.1 extracted from

  • Fujii, T.; Takaya, N.
    Denitrification by the fungus Fusarium oxysporum involves NADH-nitrate reductase (2008), Biosci. Biotechnol. Biochem., 72, 412-420.
    View publication on PubMed
Search for more literature for 1.7.1.1

Cloned(Commentary)

Cloned (Comment) Organism
gene niaD, DNA and amino acid sequence determination and analysis, functional complemention of the defective NO3- assimilation by mutant strain M10, expression in strain T1 Fusarium oxysporum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nitrate + NADH + H+ Fusarium oxysporum the enzyme is involved in the reduction of NO3- to nitrite at the initial step of denitrification nitrite + NAD+ + H2O
-
 ?
nitrate + NADH + H+ Fusarium oxysporum JCM 11502 the enzyme is involved in the reduction of NO3- to nitrite at the initial step of denitrification nitrite + NAD+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Fusarium oxysporum B1B5E3 fragment; strain JCM11502, gene niaD
-
Fusarium oxysporum B1B5E4 fragment; strain JCM11502, gene niaD
-
Fusarium oxysporum JCM 11502 B1B5E3 fragment; strain JCM11502, gene niaD
-
Fusarium oxysporum JCM 11502 B1B5E4 fragment; strain JCM11502, gene niaD
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 enzyme activity in cells under different culture conditions, overview Fusarium oxysporum
10.5
-
 cells in presence of NO3- and under O2-limitation Fusarium oxysporum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nitrate + NADH + H+ the enzyme is involved in the reduction of NO3- to nitrite at the initial step of denitrification Fusarium oxysporum nitrite + NAD+ + H2O
-
 ?
nitrate + NADH + H+ the enzyme is involved in the reduction of NO3- to nitrite at the initial step of denitrification Fusarium oxysporum JCM 11502 nitrite + NAD+ + H2O
-
 ?

Synonyms

Synonyms Comment Organism
NADH-Nar
-
 Fusarium oxysporum
NADH-nitrate reductase
-
 Fusarium oxysporum
NADH-NO3- reductase
-
 Fusarium oxysporum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Fusarium oxysporum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Fusarium oxysporum

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Fusarium oxysporum