Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.7.1.1 extracted from

  • Reda, M.; Klobus, G.
    Modifications of the activity of nitrate reductase from cucumber roots (2006), Biol. Plant., 50, 42-47.
No PubMed abstract available
Search for more literature for 1.7.1.1

Inhibitors

Inhibitors Comment Organism Structure
ATP at low pH, the presence of ATP alone in the incubation medium is sufficient to inactivate nitrate reductase Cucumis sativus
Mg2+ 5 mM MgCl2 decreases enzyme activity at pH 7.5, this effect is completely reversed by the addition of EDTA into samples returning the enzyme's activity to its initial level Cucumis sativus
additional information An inhibitory effect of Mg-ATP is cancelled in the presence of staurosporine (the protein kinase inhibitor) and completely reversed after addition of EDTA as well as AMP Cucumis sativus

Metals/Ions

Metals/Ions Comment Organism Structure
EDTA 5 mM, 27% increase of activity at pH 7.5 Cucumis sativus

Organism

Organism UniProt Comment Textmining
Cucumis sativus
-
 cultivar Wisconsin
-

Purification (Commentary)

Purification (Comment) Organism
Sephadex G-25 gel filtration Cucumis sativus

Source Tissue

Source Tissue Comment Organism Textmining
root
-
 Cucumis sativus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nitrate + NADH
-
 Cucumis sativus nitrite + NAD+ + H2O
-
 ?

Synonyms

Synonyms Comment Organism
NADH-dependent nitrate reductase
-
 Cucumis sativus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Cucumis sativus

pH Stability

pH Stability pH Stability Maximum Comment Organism
additional information
-
 activity determined at pH 6.0 was significantly lower than at pH 7.5 Cucumis sativus

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Cucumis sativus