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Literature summary for 1.7.1.1 extracted from

  • De La Rosa, F.F.; Castillo, F.; Palacian, E.
    Effects of denaturing agents on spinach nitrate reductase (1977), Phytochemistry, 16, 875-879.
No PubMed abstract available
Search for more literature for 1.7.1.1

General Stability

General Stability Organism
0.03% SDS produces almost complete inactivation of NADH-diaphorase and NADH-nitrate reductase, while FNH2-nitrate reductase retains 60% of the original activity, FAD has no protecting effect Spinacia oleracea
1 M guanidine hydrochloride, in presence of FAD, 80% inactivation of FNH2-nitrate reductase activity and NADH-nitrate reductase activity, NADH-diaphorase activity is unaffected Spinacia oleracea
4 M urea, in presence of FAD, inactivation of FMNH2-nitrate reductase and NADH-nitrate reductase activity, only a slight effect on the NADH-diaphorase activity Spinacia oleracea

Organism

Organism UniProt Comment Textmining
Spinacia oleracea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Spinacia oleracea

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Spinacia oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
FMNH2 + nitrate
-
 Spinacia oleracea FMN + nitrite
-
 ?
additional information NADH-diaphorase activity Spinacia oleracea ?
-
 ?
nitrate + NADH
-
 Spinacia oleracea nitrite + NAD+ + H2O
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
 20 min, in absence of FAD, stable Spinacia oleracea

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 8.5 1 h, stable Spinacia oleracea

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Spinacia oleracea