Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Bromophenol blue | noncompetitive versus NADH | Cucurbita sp. | |
p-hydroxymercuribenzoate | inactivation of NADH:nitrate reductase activity, no loss of bromphenol blue: nitrate reductase activity | Cucurbita sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
Bromophenol blue | at 10 mM nitrate | Cucurbita sp. | |
0.5 | - |
nitrate | at 0.2 mM bromophenol blue | Cucurbita sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cucurbita sp. | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
bromophenol blue + nitrate | - |
Cucurbita sp. | ? + nitrite | - |
? | |
nitrate + NADH | - |
Cucurbita sp. | nitrite + NAD+ + H2O | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
40 min, 50% loss of nitrate reductase activity, slight increase of bromphenol blue activity | Cucurbita sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Cucurbita sp. |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
Bromophenol blue | - |
Cucurbita sp. |