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Literature summary for 1.6.5.6 extracted from

  • Zhang, J.J.; Liu, H.; Xiao, Y.; Zhang, X.E.; Zhou, N.Y.
    Identification and characterization of catabolic para-nitrophenol 4-monooxygenase and para-benzoquinone reductase from Pseudomonas sp. strain WBC-3 (2009), J. Bacteriol., 191, 2703-2710.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.6.5.6

Cloned(Commentary)

Cloned (Comment) Organism
expressed as His-tagged fusion protein in Escherichia coli Pseudomonas sp. WBC-3

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0067
-
 p-benzoquinone
-
 Pseudomonas sp. WBC-3
0.181
-
 NADPH
-
 Pseudomonas sp. WBC-3

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
 SDS-PAGE Pseudomonas sp. WBC-3

Organism

Organism UniProt Comment Textmining
Pseudomonas sp. WBC-3 C1I202
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni2+-NTA affinity chromatography Pseudomonas sp. WBC-3

Storage Stability

Storage Stability Organism
-70°C, 50% glycerol, 1 month, no loss of activity Pseudomonas sp. WBC-3
0°, on ice, 12 h, fairly stable Pseudomonas sp. WBC-3

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NADPH + H+ + p-benzoquinone
-
 Pseudomonas sp. WBC-3 NADP+ + hydroquinone
-
 ?

Subunits

Subunits Comment Organism
dimer SDS-PAGE, 2 * 25000 Da Pseudomonas sp. WBC-3

Synonyms

Synonyms Comment Organism
p-Benzoquinone reductase
-
 Pseudomonas sp. WBC-3
PnpB
-
 Pseudomonas sp. WBC-3

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Pseudomonas sp. WBC-3

Cofactor

Cofactor Comment Organism Structure
FAD FMN is preferred to FAD Pseudomonas sp. WBC-3
FMN enzyme is flavin mononucleotide-dependent, FMN is preferred to FAD Pseudomonas sp. WBC-3
NADPH enzyme is NADPH-dependent Pseudomonas sp. WBC-3

General Information

General Information Comment Organism
physiological function PnpB enhances the p-nitrophenol degradation by p-nitrophenol 4-monooxygenase Pseudomonas sp. WBC-3