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Literature summary for 1.6.5.5 extracted from

  • Hervas, M.; Lopez-Maury, L.; Leon, P.; Sanchez-Riego, A.M.; Florencio, F.J.; Navarro, J.A.
    ArsH from the cyanobacterium Synechocystis sp. PCC 6803 is an efficient NADPH-dependent quinone reductase (2012), Biochemistry, 51, 1178-1187.
    View publication on PubMed
Search for more literature for 1.6.5.5

Cloned(Commentary)

Cloned (Comment) Organism
gene arsH Synechocystis sp.

Inhibitors

Inhibitors Comment Organism Structure
menadione
-
 Synechocystis sp.
NADP+ can act as a competitive inhibitor for NADPH binding at the active site of an enzyme Synechocystis sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information stopped-flow and laser-flash photolysis kinetic analyses, steady-state kinetics Synechocystis sp.
0.0012
-
 dibromothymoquinone pH 7.5, 10°C Synechocystis sp.
0.0013
-
 2,5-dimethyl-4-benzoquinone pH 7.5, 10°C Synechocystis sp.
0.0028
-
 duroquinone pH 7.5, 10°C Synechocystis sp.
0.0057
-
 menadione pH 7.5, 10°C Synechocystis sp.
0.0119
-
 coenzyme Q10 pH 7.5, 10°C Synechocystis sp.
0.0237
-
 2-Hydroxy-1,4-naphthoquinone pH 7.5, 10°C Synechocystis sp.
0.0315
-
 NADPH pH 7.5, 10°C Synechocystis sp.
0.0345
-
 anthraquinone-2-sulfonate pH 7.5, 10°C Synechocystis sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25900
-
 4 * 25900, SDS-PAGE Synechocystis sp.
108000
-
 gel filtration Synechocystis sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NADPH + H+ + 2 quinone Synechocystis sp.
-
 NADP+ + 2 semiquinone
-
 ?

Organism

Organism UniProt Comment Textmining
Synechocystis sp.
-
 gene arsh, encoded in the arsenic resistance operon
-

Reaction

Reaction Comment Organism Reaction ID
NADPH + H+ + 2 quinone = NADP+ + 2 semiquinone ping pong reaction mechanism Synechocystis sp.
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information although the enzyme is able to stabilize the anionic semiquinone form of the FMN, reduction of quinones involves the hydroquinone form of the flavin cofactor, and the enzymatic reaction occurs through a ping pong-type mechanism. ArsH is able to catalyze one-electron reactions (oxygen and cytocrome c reduction), involving the FMN semiquinone form, but with lower efficiency Synechocystis sp. ?
-
 ?
NADPH + H+ + 2 2,5-dimethyl-4-benzoquinone
-
 Synechocystis sp. NADP+ + 2 2,5-dimethyl-4-benzosemiquinone
-
 ?
NADPH + H+ + 2 2-hydroxy-1,4-naphthoquinone
-
 Synechocystis sp. NADP+ + 2 2-hydroxy-1,4-naphthosemiquinone
-
 ?
NADPH + H+ + 2 anthraquinone-2-sulfonate
-
 Synechocystis sp. NADP+ + ?
-
 ?
NADPH + H+ + 2 coenzyme Q10
-
 Synechocystis sp. NADP+ + ?
-
 ?
NADPH + H+ + 2 dibromothymoquinone
-
 Synechocystis sp. NADP+ + 2 dibromothymosemiquinone
-
 ?
NADPH + H+ + 2 duroquinone
-
 Synechocystis sp. NADP+ + 2 durosemiquinone
-
 ?
NADPH + H+ + 2 menadione
-
 Synechocystis sp. NADP+ + ?
-
 ?
NADPH + H+ + 2 quinone
-
 Synechocystis sp. NADP+ + 2 semiquinone
-
 ?
NADPH + H+ + 2 quinone the catalytic cycle of ArsH consists of the acceptance of two electrons from NADPH to reduce the flavin cofactor (reductive half-reaction) and the transfer of these electrons to an acceptor (oxidative half-reaction) Synechocystis sp. NADP+ + 2 semiquinone
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 25900, SDS-PAGE Synechocystis sp.

Synonyms

Synonyms Comment Organism
ArsH
-
 Synechocystis sp.
NADPH-dependent quinone reductase
-
 Synechocystis sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
10 25 assay at Synechocystis sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5
-
 coenzyme Q10 pH 7.5, 10°C Synechocystis sp.
9.4
-
 anthraquinone-2-sulfonate pH 7.5, 10°C Synechocystis sp.
11
-
 2-Hydroxy-1,4-naphthoquinone pH 7.5, 10°C Synechocystis sp.
25
-
 duroquinone pH 7.5, 10°C Synechocystis sp.
26
-
 menadione pH 7.5, 10°C Synechocystis sp.
35
-
 dibromothymoquinone pH 7.5, 10°C Synechocystis sp.
36.7
-
 2,5-dimethyl-4-benzoquinone pH 7.5, 10°C Synechocystis sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8 assay at Synechocystis sp.

Cofactor

Cofactor Comment Organism Structure
FMN in silico structural model of ArsH reconstituted with FMN, overview Synechocystis sp.

General Information

General Information Comment Organism
additional information in silico structural model of ArsH reconstituted with FMN, overview Synechocystis sp.
physiological function ArsH plays a role in the response to oxidative stress caused by arsenite Synechocystis sp.