General Stability | Organism |
---|---|
NADPH protects against inactivation caused by heat, NEM or H2O2 | Cavia porcellus |
very stable to freezing | Cavia porcellus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoate) | - |
Cavia porcellus | |
Cibacron blue 3GA | - |
Cavia porcellus | |
dicoumarol | - |
Cavia porcellus | |
H2O2 | - |
Cavia porcellus | |
additional information | no inactivation by 0.5 mM iodoacetate | Cavia porcellus | |
NADP+ | - |
Cavia porcellus | |
NEM | - |
Cavia porcellus | |
Nitrofurantoin | - |
Cavia porcellus | |
p-chloromercuriphenylsulfonate | - |
Cavia porcellus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
NADPH | - |
Cavia porcellus | |
0.01 | - |
9,10-phenanthrenequinone | - |
Cavia porcellus | |
0.027 | - |
5-hydroxy-1,4-naphthoquinone | - |
Cavia porcellus | |
0.143 | - |
1,4-benzoquinone | - |
Cavia porcellus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cavia porcellus | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
NADPH + H+ + 2 quinone = NADP+ + 2 semiquinone | enzyme acts through a one-electron transfer process | Cavia porcellus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
lens | - |
Cavia porcellus | - |
Storage Stability | Organism |
---|---|
-20°C, less than 10% loss of activity after 4-6 weeks | Cavia porcellus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-naphthoquinone + NADPH | - |
Cavia porcellus | 1,2-naphthoquinol + NADP+ | - |
? | |
1,4-benzoquinone + NADPH | 15.3% of the activity with 1,2-naphthoquinone | Cavia porcellus | 1,4-benzosemiquinone + NADP+ | - |
? | |
1,4-naphthoquinone + NADPH + H+ | 2.6% of the activity with 1,2-naphthoquinone | Cavia porcellus | 1,4-naphthosemiquinone + NADP+ | - |
? | |
5-hydroxy-1,4-naphthoquinone + NADPH | i.e. juglone, 12.5% of the activity with 1,2-naphthoquinone | Cavia porcellus | 5-hydroxy-1,4-naphthosemiquinone + NADP+ | - |
? | |
5-hydroxy-2-methyl-1,4-naphthoquinone + NADPH | i.e. plumbagin, 0.9% of the activity with 1,2-naphthoquinone | Cavia porcellus | 5-hydroxy-2-methyl-1,4-naphthoquinol + NADP+ | - |
? | |
9,10-phenanthrenequinone + NADPH | 50% of the activity with 1,2-naphthoquinone | Cavia porcellus | 9,10-phenanthrenesemiquinone + NADP+ | - |
? | |
methyl-1,4-benzoquinone + NADPH | 20.6% of the activity with 1,2-naphthoquinone | Cavia porcellus | methyl-1,4-benzoquinol + NADP+ | - |
? | |
additional information | inactive with: menadione, ubiquinone, 9,10-anthraquinone, vitamin K1, vitamin K2 | Cavia porcellus | ? | - |
? | |
NADPH + H+ + oxidized 2,6-dichlorophenolindophenol | 3.8% of the activity with 1,2-naphthoquinone | Cavia porcellus | NADP+ + reduced 2,6-dichlorophenolindophenol | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
10 min, 65% loss of activity | Cavia porcellus |
51 | - |
10 min, complete loss of activity | Cavia porcellus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.6 | - |
5-hydroxy-1,4-naphthoquinone | - |
Cavia porcellus | |
3.4 | - |
1,4-benzoquinone | - |
Cavia porcellus | |
10 | - |
9,10-phenanthrenequinone | - |
Cavia porcellus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | specific for | Cavia porcellus |