Literature summary for 1.6.5.4 extracted from

Park, A.; Kim, I.; Do, H.; Jeon, B.; Lee, C.; Roh, S.; Shin, S.; Park, H.; Kim, Y.; Kim, Y.; Yoon, H.; Lee, J.; Kim, H.
Structure and catalytic mechanism of monodehydroascorbate reductase, MDHAR, from Oryza sativa L. japonica (2016), Sci. Rep., 6, 33903 .

Cloned(Commentary)

Cloned (Comment)	Organism
-	Oryza sativa Japonica Group

Crystallization (Commentary)

Crystallization (Comment)	Organism
structures in the presence of cofactors, NAD+ and NADP+, and complexed with ascorbic acid and isoascorbic acid. The overall structure of MDHAR is similar to other iron-sulfur protein reductases, except for a unique long loop of 63-80 residues, which seems to be essential in forming the active site pocket. Residue Arg320 plays a major substrate binding role, and Tyr349 mediates electron transfer from NAD(P)H to bound substrate via FAD. The enzymatic activity favours NADH as an electron donor over NADPH	Oryza sativa Japonica Group

Crystallization (Comment)

Organism

structures in the presence of cofactors, NAD+ and NADP+, and complexed with ascorbic acid and isoascorbic acid. The overall structure of MDHAR is similar to other iron-sulfur protein reductases, except for a unique long loop of 63-80 residues, which seems to be essential in forming the active site pocket. Residue Arg320 plays a major substrate binding role, and Tyr349 mediates electron transfer from NAD(P)H to bound substrate via FAD. The enzymatic activity favours NADH as an electron donor over NADPH

Oryza sativa Japonica Group

Protein Variants

Protein Variants	Comment	Organism
C70A	about 105% of wild-type activity	Oryza sativa Japonica Group
E196A	residue E196 forms a hydrogen bond to the O2B molecule in the adenosine ring of NAD. Mutation reduces catalytic activity. Mutant E196A has only about 3 times higher affinity for NAD than that for NADP, i.e. 16fold increase in affinity for NADP compared to the wild-type	Oryza sativa Japonica Group
G72N	about 70% of wild-type activity	Oryza sativa Japonica Group
R320A	about 20% of wild-type activity	Oryza sativa Japonica Group
Y349A	about 10% of wild-type activity	Oryza sativa Japonica Group
Y349F	about 10% of wild-type activity	Oryza sativa Japonica Group
Y349W	about 10% of wild-type activity	Oryza sativa Japonica Group

Organism

Organism	UniProt	Comment	Textmining
Oryza sativa Japonica Group	Q652L6	-	-

Synonyms

Synonyms	Comment	Organism
Os09g0567300	-	Oryza sativa Japonica Group

Cofactor

Cofactor	Comment	Organism	Structure
NADH	-	Oryza sativa Japonica Group
NADPH	about 50% of the activity with NADH	Oryza sativa Japonica Group