Cloned (Comment) | Organism |
---|---|
gene MDHAR3, functional overexpression in transgenic Solanum lycopersicum plant leaves, quantitative reverse-transcriptase PCR expression analysis | Solanum lycopersicum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | transgenic lines overexpressing MDHAR show a decrease in ascorbate levels in leaves, whereas lines where MDHAR is silenced show an increase in these levels in both fruits and leave. In all three lines where MDHAR3 is silenced (MDS lines), a significant decrease in overall MDHAR activity is observed in leavess. Transcript levels of MDHAR1 (SGN-U584073) and MDHAR2 (SGN-U583672) remain unaffected by modification of MDHAR3 | Solanum lycopersicum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Solanum lycopersicum | 5829 | - |
additional information | MDHAR isoforms in both the cytosol and peroxisome | Solanum lycopersicum | - |
- |
peroxisome | isozyme MDHAR3 | Solanum lycopersicum | 5777 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADH + H+ + 2 monodehydroascorbate | Solanum lycopersicum | - |
NAD+ + 2 ascorbate | - |
? | |
NADH + H+ + 2 monodehydroascorbate | Solanum lycopersicum West Virginia 106 | - |
NAD+ + 2 ascorbate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solanum lycopersicum | - |
- |
- |
Solanum lycopersicum West Virginia 106 | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | - |
Solanum lycopersicum | - |
leaf | - |
Solanum lycopersicum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADH + H+ + 2 monodehydroascorbate | - |
Solanum lycopersicum | NAD+ + 2 ascorbate | - |
? | |
NADH + H+ + 2 monodehydroascorbate | - |
Solanum lycopersicum West Virginia 106 | NAD+ + 2 ascorbate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MDHAR | - |
Solanum lycopersicum |
monodehydroascorbate reductase | - |
Solanum lycopersicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Solanum lycopersicum |
General Information | Comment | Organism |
---|---|---|
evolution | in plants, MDHAR belongs to a multigene family | Solanum lycopersicum |
malfunction | transgenic lines overexpressing MDHAR show a decrease in ascorbate levels in leaves, whereas lines where MDHAR is silenced show an increase in these levels in both fruits and leaves | Solanum lycopersicum |
metabolism | the enzyme is involved in the ascorbate recycling pathway, overview | Solanum lycopersicum |
physiological function | the enzyme negatively regulates ascorbate levels in Solanum lycopersicum, isozyme MDHAR3 activity has a negative impact on ascorbate levels in both leaves and fruits | Solanum lycopersicum |