Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.6.5.4 extracted from

  • Bando, M.; Inoue, T.; Oka, M.; Nakamura, K.; Kawai, K.; Obazawa, H.; Kobayashi, S.; Takehana, M.
    Isolation of ascorbate free radical reductase from rabbit lens soluble fraction (2004), Exp. Eye Res., 79, 869-873.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0006
-
NADH with substrate monodehydroascorbate Oryctolagus cuniculus
0.0012
-
NADH with ferricyanide as substrate Oryctolagus cuniculus
0.0013
-
ferricyanide
-
Oryctolagus cuniculus
0.0134
-
monodehydroascorbate
-
Oryctolagus cuniculus

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Oryctolagus cuniculus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
x * 31000, SDS-PAGE Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NADH + monodehydroascorbate Oryctolagus cuniculus
-
NAD+ + ascorbate
-
?

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
several enzyme forms
-

Purification (Commentary)

Purification (Comment) Organism
several native enzyme forms from lens soluble fraction by gel filtration, affinity chromatography, native isoelectric focusing, and 2D-electrophoresis, about 1400fold Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
lens
-
Oryctolagus cuniculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ferricyanide + NADH enzyme also shows diaphorase activity, i.e. ferricyanide reductase Oryctolagus cuniculus 2 ferrocyanide + NAD+ + H+
-
?
ferricyanide + reduced 2,6-dichlorophenolindophenol enzyme also shows diaphorase activity, i.e. ferricyanide reductase Oryctolagus cuniculus ferrocyanide + 2,6-dichlorophenolindophenol
-
?
monodehydroascorbate + NADH
-
Oryctolagus cuniculus ascorbate + NAD+
-
?
NADH + monodehydroascorbate
-
Oryctolagus cuniculus NAD+ + ascorbate
-
?

Subunits

Subunits Comment Organism
? x * 31000, SDS-PAGE Oryctolagus cuniculus

Synonyms

Synonyms Comment Organism
AFR reductase
-
Oryctolagus cuniculus
ascorbate free radical reductase
-
Oryctolagus cuniculus

Cofactor

Cofactor Comment Organism Structure
NADH
-
Oryctolagus cuniculus

pI Value

Organism Comment pI Value Maximum pI Value
Oryctolagus cuniculus major enzyme form, native isoelectric focusing
-
6.9
Oryctolagus cuniculus minor enzyme forms, native isoelectric focusing
-
7