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Literature summary for 1.6.5.4 extracted from
- The isolation and characterization of a NADH: semidehydroascorbic acid oxidoreductase from Neurospora crassa (1972), Hoppe-Seyler's Z. Physiol. Chem., 353, 1931-1942.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | 1 mM, stimulates | Neurospora crassa |  |
| diphosphate | 1 mM, stimulates | Neurospora crassa | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1-(2-thenoyl)-3,3,3-trifluoracetone | 0.3 mM, about 10% inhibition | Neurospora crassa | |
| 2,2'-dipyridyl | 1 mM, about 10% inhibition | Neurospora crassa | |
| 2-Iodoacetamide | - |
Neurospora crassa | |
| citrate | 1 mM, about 10% inhibition | Neurospora crassa | |
| NEM | - |
Neurospora crassa | |
| PCMB | - |
Neurospora crassa | |
| sodium diethyldithiocarbaminate | 1 mM, about 10% inhibition | Neurospora crassa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0012 | - |
semidehydroascorbic acid | - |
Neurospora crassa | |
| 0.012 | - |
NADH | - |
Neurospora crassa | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Neurospora crassa | 5737 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 66000 | - |
gel filtration | Neurospora crassa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Neurospora crassa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Neurospora crassa |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Neurospora crassa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 ferricytochrome b5 + NADH | - |
Neurospora crassa | 2 ferrocytochrome b5 + NAD+ + H+ | - |
? | |
| 2 ferricytochrome c + NADH | - |
Neurospora crassa | 2 ferrocytochrome c + NAD+ + H+ | - |
? | |
| additional information | no activity with NADPH | Neurospora crassa | ? | - |
? | |
| NADH + 2,6-dichlorophenol indophenol | - |
Neurospora crassa | NAD+ + ? | - |
? | |
| NADH + monodehydroascorbate | - |
Neurospora crassa | NAD+ + ascorbate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 39 | - |
- |
Neurospora crassa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
14 min, complete inactivation | Neurospora crassa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.2 | - |
Neurospora crassa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.7 | 7.9 | about 50% of maximal activity at pH 6.7 and at pH 7.9 | Neurospora crassa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Neurospora crassa | |
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