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Literature summary for 1.6.5.2 extracted from

  • Kim, I.K.; Yim, H.S.; Kim, M.K.; Kim, D.W.; Kim, Y.M.; Cha, S.S.; Kang, S.O.
    Crystal structure of a new type of NADPH-dependent quinone oxidoreductase (QOR2) from Escherichia coli (2008), J. Mol. Biol., 379, 372-384.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
native enzyme and in complex with NADPH. The N-terminal domain, which adopts the Rossmann fold, provides a platform for NADPH binding, whereas the C-terminal domain, which contains a hydrophobic pocket connected to the NADPH-binding site, plays important roles in substrate binding. Asn143 near the NADPH-binding site is involved in substrate binding and catalysis Escherichia coli

Protein Variants

Protein Variants Comment Organism
N143A residue near NADPH-binding site. About 10% of wild-type activity Escherichia coli
N143L residue near NADPH-binding site. About 10% of wild-type activity Escherichia coli
W139A residue near NADPH-binding site. About 30% of wild-type activity Escherichia coli
W139F residue near NADPH-binding site. Activity comparable to wild-type Escherichia coli
W139I residue near NADPH-binding site. About 50% of wild-type activity Escherichia coli
Y140A residue near NADPH-binding site. Almost complete loss of activity Escherichia coli
Y140F residue near NADPH-binding site. Activity comparable to wild-type Escherichia coli
Y140I residue near NADPH-binding site. Almost complete loss of activity Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0625
-
NADPH mutant Y140F, pH 7.5, temperature not specified in the publication Escherichia coli
0.0656
-
NADPH wild-type, pH 7.5, temperature not specified in the publication Escherichia coli
0.0915
-
NADPH mutant W139F, pH 7.5, temperature not specified in the publication Escherichia coli
0.195
-
NADPH mutant W139I, pH 7.5, temperature not specified in the publication Escherichia coli
0.225
-
NADPH mutant W139A, pH 7.5, temperature not specified in the publication Escherichia coli
0.395
-
NADPH mutant N143A, pH 7.5, temperature not specified in the publication Escherichia coli
0.403
-
NADPH mutant N143L, pH 7.5, temperature not specified in the publication Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P39315
-
-

Reaction

Reaction Comment Organism Reaction ID
NAD(P)H + H+ + a quinone = NAD(P)+ + a hydroquinone ping-pong mechanism with substrate inhibition Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methyl-1,4-benzoquinone + NADPH + H+ two-electron mechanism Escherichia coli methyl-1,4-benzoquinol + NADP+
-
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Synonyms

Synonyms Comment Organism
QOR2
-
Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
18.7
-
NADPH mutant N143A, pH 7.5, temperature not specified in the publication Escherichia coli
25.2
-
NADPH mutant N143L, pH 7.5, temperature not specified in the publication Escherichia coli
32.3
-
NADPH mutant W139A, pH 7.5, temperature not specified in the publication Escherichia coli
34.2
-
NADPH wild-type, pH 7.5, temperature not specified in the publication Escherichia coli
37.1
-
NADPH mutant W139F, pH 7.5, temperature not specified in the publication Escherichia coli
45.2
-
NADPH mutant Y140F, pH 7.5, temperature not specified in the publication Escherichia coli
53.5
-
NADPH mutant W139I, pH 7.5, temperature not specified in the publication Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
48
-
NADPH mutant N143A, pH 7.5, temperature not specified in the publication Escherichia coli
63
-
NADPH mutant N143L, pH 7.5, temperature not specified in the publication Escherichia coli
144
-
NADPH mutant W139A, pH 7.5, temperature not specified in the publication Escherichia coli
278
-
NADPH mutant W139I, pH 7.5, temperature not specified in the publication Escherichia coli
407
-
NADPH mutant W139F, pH 7.5, temperature not specified in the publication Escherichia coli
522
-
NADPH wild-type, pH 7.5, temperature not specified in the publication Escherichia coli
727
-
NADPH mutant Y140F, pH 7.5, temperature not specified in the publication Escherichia coli