Crystallization (Comment) | Organism |
---|---|
native enzyme and in complex with NADPH. The N-terminal domain, which adopts the Rossmann fold, provides a platform for NADPH binding, whereas the C-terminal domain, which contains a hydrophobic pocket connected to the NADPH-binding site, plays important roles in substrate binding. Asn143 near the NADPH-binding site is involved in substrate binding and catalysis | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
N143A | residue near NADPH-binding site. About 10% of wild-type activity | Escherichia coli |
N143L | residue near NADPH-binding site. About 10% of wild-type activity | Escherichia coli |
W139A | residue near NADPH-binding site. About 30% of wild-type activity | Escherichia coli |
W139F | residue near NADPH-binding site. Activity comparable to wild-type | Escherichia coli |
W139I | residue near NADPH-binding site. About 50% of wild-type activity | Escherichia coli |
Y140A | residue near NADPH-binding site. Almost complete loss of activity | Escherichia coli |
Y140F | residue near NADPH-binding site. Activity comparable to wild-type | Escherichia coli |
Y140I | residue near NADPH-binding site. Almost complete loss of activity | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0625 | - |
NADPH | mutant Y140F, pH 7.5, temperature not specified in the publication | Escherichia coli | |
0.0656 | - |
NADPH | wild-type, pH 7.5, temperature not specified in the publication | Escherichia coli | |
0.0915 | - |
NADPH | mutant W139F, pH 7.5, temperature not specified in the publication | Escherichia coli | |
0.195 | - |
NADPH | mutant W139I, pH 7.5, temperature not specified in the publication | Escherichia coli | |
0.225 | - |
NADPH | mutant W139A, pH 7.5, temperature not specified in the publication | Escherichia coli | |
0.395 | - |
NADPH | mutant N143A, pH 7.5, temperature not specified in the publication | Escherichia coli | |
0.403 | - |
NADPH | mutant N143L, pH 7.5, temperature not specified in the publication | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P39315 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
NAD(P)H + H+ + a quinone = NAD(P)+ + a hydroquinone | ping-pong mechanism with substrate inhibition | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methyl-1,4-benzoquinone + NADPH + H+ | two-electron mechanism | Escherichia coli | methyl-1,4-benzoquinol + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
QOR2 | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
18.7 | - |
NADPH | mutant N143A, pH 7.5, temperature not specified in the publication | Escherichia coli | |
25.2 | - |
NADPH | mutant N143L, pH 7.5, temperature not specified in the publication | Escherichia coli | |
32.3 | - |
NADPH | mutant W139A, pH 7.5, temperature not specified in the publication | Escherichia coli | |
34.2 | - |
NADPH | wild-type, pH 7.5, temperature not specified in the publication | Escherichia coli | |
37.1 | - |
NADPH | mutant W139F, pH 7.5, temperature not specified in the publication | Escherichia coli | |
45.2 | - |
NADPH | mutant Y140F, pH 7.5, temperature not specified in the publication | Escherichia coli | |
53.5 | - |
NADPH | mutant W139I, pH 7.5, temperature not specified in the publication | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
48 | - |
NADPH | mutant N143A, pH 7.5, temperature not specified in the publication | Escherichia coli | |
63 | - |
NADPH | mutant N143L, pH 7.5, temperature not specified in the publication | Escherichia coli | |
144 | - |
NADPH | mutant W139A, pH 7.5, temperature not specified in the publication | Escherichia coli | |
278 | - |
NADPH | mutant W139I, pH 7.5, temperature not specified in the publication | Escherichia coli | |
407 | - |
NADPH | mutant W139F, pH 7.5, temperature not specified in the publication | Escherichia coli | |
522 | - |
NADPH | wild-type, pH 7.5, temperature not specified in the publication | Escherichia coli | |
727 | - |
NADPH | mutant Y140F, pH 7.5, temperature not specified in the publication | Escherichia coli |