Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-chloromercuribenzoate | 74% residual activity at 0.15 mM | Beta vulgaris | |
ADP | 86% residual activity at 0.2 mM | Beta vulgaris | |
ATP | 91% residual activity at 0.2 mM | Beta vulgaris | |
dicumarol | most potent inhibitor, 12% residual activity at 0.15 mM | Beta vulgaris | |
EGTA | 95% residual activity at 5 mM | Beta vulgaris | |
Mersalyl | 68% residual activity at 0.15 mM | Beta vulgaris | |
additional information | insensitive to rotenone, Ca2+, platanetin and flavone have no effect on the NADH:2,3-dimethoxy-5-methyl-1,4-benzoquinone reductase activity of the purified enzyme | Beta vulgaris | |
NAD+ | 96% residual activity at 0.2 mM | Beta vulgaris | |
NADP+ | 95% residual activity at 0.2 mM | Beta vulgaris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0065 | - |
ubiquinone-0 | in 10 mM bis-Tris-propane buffer (pH 7.2), at 25°C | Beta vulgaris | |
0.34 | - |
NADH | in 10 mM bis-Tris-propane buffer (pH 7.2), at 25°C | Beta vulgaris |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Beta vulgaris | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
2 * 43000, SDS-PAGE | Beta vulgaris |
86000 | - |
gel filtration | Beta vulgaris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Beta vulgaris | - |
- |
- |
Purification (Comment) | Organism |
---|---|
blue affinity column chromatography, Resource Q column chromatography, and Superdex 200 gel filtration | Beta vulgaris |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | - |
Beta vulgaris | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ferricyanide + NADH | 60% activity compared to the reactions of NADPH or NADH with 2,3-dimethoxy-5-methyl-1,4-benzoquinone | Beta vulgaris | 2 ferrocyanide + NAD+ + H+ | - |
? | |
deamino-NADH + H+ + 2,3-dimethoxy-5-methyl-1,4-benzoquinone | 45% activity compared to NADPH or NADH | Beta vulgaris | deamino-NAD+ + 2,3-dimethoxy-5-methyl-1,4-benzoquinol | - |
? | |
NADH + H+ + 2,3-dimethoxy-5-methyl-1,4-benzoquinone | - |
Beta vulgaris | NAD+ + 2,3-dimethoxy-5-methyl-1,4-benzoquinol | - |
? | |
NADH + H+ + ubiquinone-0 | - |
Beta vulgaris | NAD+ + ubiquinol-0 | - |
? | |
NADPH + H+ + 2,3-dimethoxy-5-methyl-1,4-benzoquinone | 100% activity | Beta vulgaris | NADP+ + 2,3-dimethoxy-5-methyl-1,4-benzoquinol | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 43000, SDS-PAGE | Beta vulgaris |
Synonyms | Comment | Organism |
---|---|---|
Internal NADH dehydrogenase | - |
Beta vulgaris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Beta vulgaris |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7.5 | the enzyme maintains greater than 90% of its maximal activity between pH 5.5 and 7.5 | Beta vulgaris |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Beta vulgaris | |
NADH | - |
Beta vulgaris | |
NADPH | - |
Beta vulgaris |