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Literature summary for 1.6.5.2 extracted from
- Functions of flavin reductase and quinone reductase in 2,4,6-trichlorophenol degradation by Cupriavidus necator JMP134 (2008), J. Bacteriol., 190, 1615-1619.
Application
| Application | Comment | Organism |
|---|---|---|
| degradation | TcpB is acting as a quinone reductase for 6-chlorohydroxyquinone reduction during 2,4,6-trichlorophenol degradation, a toxic pollutant | Cupriavidus pinatubonensis JMP134 |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into the pET30-LIC vector, overexpressed in Escherichia coli BL21(DE3) | Cupriavidus pinatubonensis JMP134 |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0096 | - |
FMN | - |
Cupriavidus pinatubonensis JMP134 |  |
| 0.0863 | - |
menadione | - |
Cupriavidus pinatubonensis JMP134 | |
| 0.2311 | - |
NADH | - |
Cupriavidus pinatubonensis JMP134 | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 21000 | - |
2 * 21000, SDS-PAGE | Cupriavidus pinatubonensis JMP134 |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cupriavidus pinatubonensis JMP134 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purified as a C-terminal His tag fusion by ultracentrifugation and gel filtration, to apparent homogeneity | Cupriavidus pinatubonensis JMP134 |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.5 | - |
with FMN as substrate | Cupriavidus pinatubonensis JMP134 |
| 8.1 | - |
with ferricyanide and FAD as substrates | Cupriavidus pinatubonensis JMP134 |
| 35.3 | - |
with menadione and FAD as substrates | Cupriavidus pinatubonensis JMP134 |
| 179.1 | - |
with ferricyanide and FMN as substrates | Cupriavidus pinatubonensis JMP134 |
| 911.4 | - |
with menadione and FMN as substrates | Cupriavidus pinatubonensis JMP134 |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, KPi buffer, 10% glycerol, several months | Cupriavidus pinatubonensis JMP134 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-chloro-6-hydroxy-1,4-benzoquinone + NADH | - |
Cupriavidus pinatubonensis JMP134 | 6-chlorobenzene-1,2,4-triol + NAD+ | - |
? | |
| FAD + NADH | - |
Cupriavidus pinatubonensis JMP134 | ? | - |
? | |
| ferricyanide + FAD + NADH | - |
Cupriavidus pinatubonensis JMP134 | ? | - |
? | |
| ferricyanide + FMN + NADH | - |
Cupriavidus pinatubonensis JMP134 | ? | - |
? | |
| FMN + NADH | - |
Cupriavidus pinatubonensis JMP134 | ? | - |
? | |
| menadione + FAD + NADH | - |
Cupriavidus pinatubonensis JMP134 | ? | - |
? | |
| menadione + FMN + NADH | - |
Cupriavidus pinatubonensis JMP134 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 21000, SDS-PAGE | Cupriavidus pinatubonensis JMP134 |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Quinone reductase | - |
Cupriavidus pinatubonensis JMP134 |
| TcpB | - |
Cupriavidus pinatubonensis JMP134 |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 350.7 | - |
menadione | - |
Cupriavidus pinatubonensis JMP134 | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Cupriavidus pinatubonensis JMP134 | |
| FMN | - |
Cupriavidus pinatubonensis JMP134 | |
| NADH | - |
Cupriavidus pinatubonensis JMP134 | |
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