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Literature summary for 1.6.5.2 extracted from

  • Hong, Y.; Wang, G.; Maier, R.J.
    The NADPH quinone reductase MdaB confers oxidative stress resistance to Helicobacter hepaticus (2008), Microb. Pathog., 44, 169-174.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.6.5.2

Cloned(Commentary)

Cloned (Comment) Organism
into pET21a, His-tagged version overexpressed in Escherichia coli BL21 Origami competent cells Helicobacter hepaticus

Protein Variants

Protein Variants Comment Organism
additional information mdaB mutant can be grown in microaerophilic conditions. The mutant is more sensitive to oxidative stress reagents such as H2O2, cumene hydroperoxide, t-butyl hydroperoxide, and paraquat. Protein (migration mass of about 26 kDa) is significantly upexpressed in the mutant compared to the wild-type Helicobacter hepaticus

Organism

Organism UniProt Comment Textmining
Helicobacter hepaticus
-
 strain ATCC51449
-
Helicobacter hepaticus ATCC 51449
-
 strain ATCC51449
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 quinone or quinone-like compounds reduction assay with a specific activity ranging from 0.92-4.6 U/mg of protein Helicobacter hepaticus
4
-
 menadione-cytochrome c reduction assay Helicobacter hepaticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,4-naphthoquinone + NAD(P)H
-
 Helicobacter hepaticus 1,4-naphthohydroquinone + NAD(P)+
-
 ?
1,4-naphthoquinone + NAD(P)H
-
 Helicobacter hepaticus ATCC 51449 1,4-naphthohydroquinone + NAD(P)+
-
 ?
2 ferricyanide + NAD(P)H
-
 Helicobacter hepaticus 2 ferrocyanide + NAD(P)+ + H+
-
 ?
2 ferricyanide + NAD(P)H
-
 Helicobacter hepaticus ATCC 51449 2 ferrocyanide + NAD(P)+ + H+
-
 ?
coenzyme Q0 + NAD(P)H
-
 Helicobacter hepaticus reduced coenzyme Q0 + NADP+
-
 ?
coenzyme Q0 + NAD(P)H
-
 Helicobacter hepaticus ATCC 51449 reduced coenzyme Q0 + NADP+
-
 ?
coenzyme Q1 + NAD(P)H
-
 Helicobacter hepaticus reduced coenzyme Q1 + NADP+
-
 ?
coenzyme Q1 + NAD(P)H
-
 Helicobacter hepaticus ATCC 51449 reduced coenzyme Q1 + NADP+
-
 ?
menadione + NAD(P)H
-
 Helicobacter hepaticus reduced menadione + NADP+
-
 ?
NAD(P)H + H+ + quinone
-
 Helicobacter hepaticus NAD(P)+ + hydroquinone
-
 ?
oxidized dichlorophenol-indophenol + NAD(P)H
-
 Helicobacter hepaticus reduced dichlorophenol-indophenol + NADP+
-
 ?
oxidized dichlorophenol-indophenol + NAD(P)H
-
 Helicobacter hepaticus ATCC 51449 reduced dichlorophenol-indophenol + NADP+
-
 ?

Subunits

Subunits Comment Organism
? x * 21-22000, SDS-PAGE Helicobacter hepaticus

Synonyms

Synonyms Comment Organism
MdaB
-
 Helicobacter hepaticus
NADPH quinone reductase
-
 Helicobacter hepaticus

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Helicobacter hepaticus
NADPH
-
 Helicobacter hepaticus