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Literature summary for 1.6.5.2 extracted from

  • Patridge, E.V.; Ferry, J.G.
    WrbA from Escherichia coli and Archaeoglobus fulgidus is an NAD(P)H:quinone oxidoreductase (2006), J. Bacteriol., 188, 3498-3506.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.6.5.2

Protein Variants

Protein Variants Comment Organism
additional information enzyme knockout mutant, no phenotypes, but N-trichloromethyl-mercapto-4-cyclohexen-1,2-dicarboximide and 8-hydroxyquinoline significantly inhibit growth of mutant relative to growth of wild-type Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0058
-
 1,4-benzoquinone pH 7.2, 37°C Escherichia coli
0.014
-
 NADH pH 7.2, 37°C Escherichia coli
0.019
-
 NADH pH 7.2, 37°C Archaeoglobus fulgidus
0.031
-
 NADPH pH 7.2, 37°C Archaeoglobus fulgidus
0.037
-
 1,4-benzoquinone pH 7.2, 37°C Archaeoglobus fulgidus
0.17
-
 NADPH pH 7.2, 37°C Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21200
-
 4 * 21200, SDS-PAGE Escherichia coli
22100
-
 2 * 22100, SDS-PAGE Archaeoglobus fulgidus
22100
-
 4 * 22100, SDS-PAGE Archaeoglobus fulgidus
52000
-
 gel filtration Archaeoglobus fulgidus
79000
-
 dynamic light scattering Escherichia coli
89000
-
 dynamic light scattering Archaeoglobus fulgidus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Escherichia coli role of enzyme in protecting against environmental stressors ?
-
 ?
additional information Archaeoglobus fulgidus role of enzyme in protecting against environmental stressors ?
-
 ?

Organism

Organism UniProt Comment Textmining
Archaeoglobus fulgidus P58795 tryptophan repressor-binding protein
-
Escherichia coli P0A8G6 tryptophan repressor-binding protein
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
50
-
 substrate [Fe(CN)6]3-, pH 7.2, 37°C Escherichia coli
160
-
 substrate 2,6-dichlorophenolindophenol, pH 7.2, 37°C Escherichia coli
430
-
 substrate menadione, pH 7.2, 37°C Escherichia coli
560
-
 substrate 1,4-naphthoquinone, pH 7.2, 37°C Escherichia coli
930
-
 substrate 2,3-dihydroxy-5-methyl-1,4-benzoquinone, pH 7.2, 37°C Escherichia coli
990
-
 substrate 1,4-benzoquinone, pH 7.2, 37°C Escherichia coli
1300
-
 substrate menadione, pH 7.2, 37°C Archaeoglobus fulgidus
1400
-
 substrate 2,6-dichlorophenolindophenol, pH 7.2, 37°C Archaeoglobus fulgidus
1400
-
 substrate [Fe(CN)6]3-, pH 7.2, 37°C Archaeoglobus fulgidus
6700
-
 substrate 2,3-dihydroxy-5-methyl-1,4-benzoquinone, pH 7.2, 37°C Archaeoglobus fulgidus
8900
-
 substrate 1,4-naphthoquinone, pH 7.2, 37°C Archaeoglobus fulgidus
25000
-
 substrate 1,4-benzoquinone, pH 7.2, 37°C Archaeoglobus fulgidus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,4-benzoquinone + NAD(P)H + H+
-
 Escherichia coli 1,4-benzoquinol + NAD(P)+
-
 ?
1,4-benzoquinone + NAD(P)H + H+
-
 Archaeoglobus fulgidus 1,4-benzoquinol + NAD(P)+
-
 ?
1,4-benzoquinone + NADH + H+
-
 Escherichia coli 1,4-benzoquinol + NAD+
-
 r
1,4-benzoquinone + NADH + H+
-
 Archaeoglobus fulgidus 1,4-benzoquinol + NAD+
-
 r
1,4-naphthoquinone + NAD(P)H
-
 Escherichia coli 1,4-naphthohydroquinone + NAD(P)+
-
 ?
1,4-naphthoquinone + NAD(P)H
-
 Archaeoglobus fulgidus 1,4-naphthohydroquinone + NAD(P)+
-
 ?
2,3-dihydroxy-5-methyl-1,4-benzoquinone + NAD(P)H
-
 Escherichia coli 2,3-dihydroxy-5-methyl-1,4-benzoquinol + NAD(P)+
-
 ?
2,3-dihydroxy-5-methyl-1,4-benzoquinone + NAD(P)H
-
 Archaeoglobus fulgidus 2,3-dihydroxy-5-methyl-1,4-benzoquinol + NAD(P)+
-
 ?
menadione + NAD(P)H
-
 Escherichia coli reduced menadione + NAD(P)+
-
 ?
menadione + NAD(P)H
-
 Archaeoglobus fulgidus reduced menadione + NAD(P)+
-
 ?
additional information role of enzyme in protecting against environmental stressors Escherichia coli ?
-
 ?
additional information role of enzyme in protecting against environmental stressors Archaeoglobus fulgidus ?
-
 ?
NAD(P)H + H+ + oxidized 2,6-dichlorophenolindophenol
-
 Escherichia coli NAD(P)+ + reduced 2,6-dichlorophenolindophenol
-
 ?
NAD(P)H + H+ + oxidized 2,6-dichlorophenolindophenol
-
 Archaeoglobus fulgidus NAD(P)+ + reduced 2,6-dichlorophenolindophenol
-
 ?
[Fe(CN)6]3- + NAD(P)H
-
 Escherichia coli [Fe(CN)6]4- + NAD(P)+
-
 ?
[Fe(CN)6]3- + NAD(P)H
-
 Archaeoglobus fulgidus [Fe(CN)6]4- + NAD(P)+
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 22100, SDS-PAGE Archaeoglobus fulgidus
tetramer 4 * 21200, SDS-PAGE Escherichia coli
tetramer 4 * 22100, SDS-PAGE Archaeoglobus fulgidus

Synonyms

Synonyms Comment Organism
WrbA
-
 Escherichia coli
WrbA
-
 Archaeoglobus fulgidus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6
-
 NADPH pH 7.2, 37°C Escherichia coli
8.9
-
 NADH pH 7.2, 37°C Escherichia coli
120
-
 NADH pH 7.2, 37°C Archaeoglobus fulgidus
170
-
 NADPH pH 7.2, 37°C Archaeoglobus fulgidus
370
-
 1,4-benzoquinone pH 7.2, 37°C Escherichia coli
3200
-
 1,4-benzoquinone pH 7.2, 37°C Archaeoglobus fulgidus

pH Range

pH Minimum pH Maximum Comment Organism
5 8.5 more than 95% of maximum activity within this range Archaeoglobus fulgidus
6 8 more than 95% of maximum activity within this range Escherichia coli

Cofactor

Cofactor Comment Organism Structure
FMN one molecule per monomer Escherichia coli
FMN one molecule per monomer Archaeoglobus fulgidus
NADH
-
 Escherichia coli
NADPH
-
 Escherichia coli