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Literature summary for 1.6.5.2 extracted from

  • Chen, S.; Clarke, P.E.; Martino, P.A.; Deng, P.S.K.; Yeh, C.H.; Lee, T.D.; Prochaska, H.J.; Talalay, P.
    Mouse liver NAD(P)H:quinone acceptor oxidoreductase: protein sequence analysis by tandem mass spectrometry, cDNA cloning, expression in Escherichia coli, and enzyme activity analysis (1994), Protein Sci., 3, 1296-1304.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.6.5.2

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Mus musculus
expression in Escherichia coli Homo sapiens
expression in Escherichia coli Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
3,3'-methylene-bis(4-hydroxycoumarin)
-
 Homo sapiens
3,3'-methylene-bis(4-hydroxycoumarin) competitive vs. NADH Mus musculus
3,3'-methylene-bis(4-hydroxycoumarin)
-
 Rattus norvegicus
5,7-dihydroxyflavone 0.009 mM, approx. 90% inhibition Homo sapiens
5,7-dihydroxyflavone 0.009 mM, approx. 20% inhibition Mus musculus
5,7-dihydroxyflavone 0.009 mM, approx. 80% inhibition Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
 menadione
-
 Homo sapiens
0.0026
-
 menadione
-
 Rattus norvegicus
0.0041
-
 menadione
-
 Mus musculus
0.12
-
 NADPH
-
 Rattus norvegicus
0.17
-
 NADPH
-
 Mus musculus
0.19
-
 NADH
-
 Rattus norvegicus
0.24
-
 NADPH
-
 Homo sapiens
0.29
-
 NADH
-
 Homo sapiens
0.32
-
 NADH
-
 Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30835
-
 2 * 30835, electrospray mass spectrometry Mus musculus
30835
-
 2 * 30835, electrospray mass spectrometry Homo sapiens
30835
-
 2 * 30835, electrospray mass spectrometry Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Mus musculus
-
 2 forms have the same primary sequence
-
Rattus norvegicus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
no glycoprotein
-
 Rattus norvegicus
no glycoprotein very unstable posttranslational modification is suggested that is not detectable by mass spectrometry Mus musculus

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-methyl-1,4-naphthoquinone + NAD(P)H
-
 Mus musculus 2-methyl-1,4-naphthoquinol + NAD(P)+
-
 ?
menadione + NADH + H+
-
 Mus musculus menadiol + NAD+
-
 r

Subunits

Subunits Comment Organism
dimer 2 * 30835, electrospray mass spectrometry Mus musculus
dimer 2 * 30835, electrospray mass spectrometry Homo sapiens
dimer 2 * 30835, electrospray mass spectrometry Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Homo sapiens
FAD
-
 Rattus norvegicus
FAD purified recombinant enzyme contains FAD whereas purified native enzyme does not contain FAD Mus musculus