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Literature summary for 1.6.5.2 extracted from
- Menadione reductase from Clostridium tyrobutyricum (1980), Can. J. Microbiol., 26, 324-329.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3,3'-methylene-bis(4-hydroxycoumarin) | - |
Clostridium tyrobutyricum |  |
| additional information | not inhibited by o-phenanthroline, KCN, quinacrine, EDTA and 2,3-dimercapto-1-propanol | Clostridium tyrobutyricum | |
| p-chloromercuribenzoate | - |
Clostridium tyrobutyricum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0025 | - |
FMN | - |
Clostridium tyrobutyricum | |
| 0.035 | - |
menadione | cofactor NADH | Clostridium tyrobutyricum | |
| 0.223 | - |
NADH | cofactor menadione | Clostridium tyrobutyricum | |
| 0.34 | - |
NADPH | acceptor menadione | Clostridium tyrobutyricum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
gel filtration | Clostridium tyrobutyricum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium tyrobutyricum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Clostridium tyrobutyricum |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| NAD(P)H + H+ + a quinone = NAD(P)+ + a hydroquinone | ping-pong mechanism | Clostridium tyrobutyricum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 46.2 | - |
- |
Clostridium tyrobutyricum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,4-naphthoquinone + NAD(P)H | - |
Clostridium tyrobutyricum | 1,4-naphthohydroquinone + NAD(P)+ | - |
? | |
| benzoquinone + 2 NAD(P)H + H+ | no activity with coenzyme Q6 and vitamin K1 | Clostridium tyrobutyricum | benzohydroquinone + 2 NAD(P)+ | - |
? | |
| menadione + NADH + H+ | - |
Clostridium tyrobutyricum | menadiol + NAD+ | - |
r | |
| NAD(P)H + H+ + oxidized 2,6-dichlorophenolindophenol | low activity | Clostridium tyrobutyricum | NAD(P)+ + reduced 2,6-dichlorophenolindophenol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 60000, SDS-PAGE | Clostridium tyrobutyricum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.4 | - |
plateau region from pH 6.0-7.5 with 70% activity | Clostridium tyrobutyricum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FMN | contains FNN as prosthetic group | Clostridium tyrobutyricum | |
| NADH | - |
Clostridium tyrobutyricum | |
| NADPH | - |
Clostridium tyrobutyricum | |
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