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Literature summary for 1.6.5.2 extracted from

  • Märki, F.; Martius, C.
    Vitamin K-Reduktase, Darstellung und eigenschaften (1960), Biochem. Z., 333, 111-135.
No PubMed abstract available
Search for more literature for 1.6.5.2

Inhibitors

Inhibitors Comment Organism Structure
2-(4'-chlorophenyl)-indan-1,3-dione 0.0002 mM, 50% inhibition Bos taurus
3,3'-methylene-bis(4-hydroxycoumarin)
-
 Bos taurus
3,3'-methylene-bis(4-hydroxycoumarin)
-
 Rattus norvegicus
3-(alpha-phenylpropyl)-4-hydroxycoumarin 0.01 mM, 50% inhibition Bos taurus
3-[alpha-(4'-nitrophenyl)-beta-acetylethyl]-4-hydroxycoumarin 0.02 mM, 50% inhibition Bos taurus
4,4'-dichlorodicoumarol 0.001 mM, 50% inhibition Bos taurus
4-Hydroxycoumarin
-
 Bos taurus
4-Methoxycoumarin
-
 Bos taurus
Dicoumarol dimethylether 0.0005, 50% inhibition Bos taurus
ethyl bis(2-hydroxy-4-oxo-4H-chromen-3-yl)acetate
-
 Bos taurus
methylene-5,5'-bis(4,6-dioxo-2-methyldihydropyran) 0.003 mM, 50% inhibition Bos taurus
additional information not inhibited by iodoacetate, 4-chloromercuribenzoate, Cu2+, FeSO4, FeCl3, CdSO4, ZnSO4, CuSO4, EDTA, alpha,alpha'-dipyridiyl, o-phenanthroline, dimethylglyoxime, dithizon, NaCN, amytal, veronal, antimycin, 2,4-dinitrophenol and thyroxin Bos taurus
additional information
-
 Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
 sedimentation equilibrium, sedimentation velocity Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
vitamin K + NAD(P)H + H+ Bos taurus
-
 reduced vitamin K + NAD(P)+
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Gallus gallus
-
-
-
Oryctolagus cuniculus
-
-
-
Rattus norvegicus
-
-
-
Sus scrofa
-
-
-
Tinca vulgaris
-
-
-
Xenopus laevis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
heart
-
 Rattus norvegicus
-
heart
-
 Bos taurus
-
heart
-
 Oryctolagus cuniculus
-
kidney
-
 Gallus gallus
-
kidney
-
 Rattus norvegicus
-
kidney
-
 Sus scrofa
-
kidney
-
 Bos taurus
-
kidney
-
 Oryctolagus cuniculus
-
liver
-
 Gallus gallus
-
liver
-
 Rattus norvegicus
-
liver
-
 Sus scrofa
-
liver
-
 Bos taurus
-
liver
-
 Oryctolagus cuniculus
-
liver
-
 Xenopus laevis
-
liver
-
 Tinca vulgaris
-
lung
-
 Rattus norvegicus
-
lung
-
 Bos taurus
-
lung
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 1400000.0, 1 unit is defined as the amount of enzyme causing a decrease in absorbance at 340 nm of 0.001/min Bos taurus

Storage Stability

Storage Stability Organism
-20°C, some weeks Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,4-dimethoxymethylbenzoquinone + NAD(P)H
-
 Bos taurus 1,4-dimethoxymethylhydrobenzoquinone + NAD(P)+
-
 ?
1,4-naphthoquinone + NAD(P)H
-
 Bos taurus 1,4-naphthohydroquinone + NAD(P)+
-
 ?
1,4-tetramethylbenzoquinone + NAD(P)H
-
 Bos taurus 1,4-tetramethylhydrobenzoquinone + NAD(P)+
-
 ?
2,3-dimethyl-1,4-naphthoquinone + NAD(P)H
-
 Bos taurus 2,3-dimethyl-1,4-hydronaphthoquinone + NAD(P)+
-
 ?
2-ethyl-1,4-naphthoquinone + NAD(P)H
-
 Bos taurus 2-ethyl-1,4-hydronaphthoquinone + NAD(P)+
-
 ?
2-methyl-1,4-naphthoquinone + NAD(P)H artificial acceptor, trivial name menadione Rattus norvegicus 2-methyl-1,4-naphthoquinol + NAD(P)+
-
 ?
2-methyl-1,4-naphthoquinone + NAD(P)H artificial acceptor, trivial name menadione Bos taurus 2-methyl-1,4-naphthoquinol + NAD(P)+
-
 ?
2-methyl-1,4-naphthoquinone + NAD(P)H
-
 Bos taurus 2-methyl-1,4-dihydronaphthoquinone + NAD(P)+
-
 ?
benzoquinone + 2 NAD(P)H + H+
-
 Bos taurus benzohydroquinone + 2 NAD(P)+
-
 ?
K3[Fe(CN)6] + NAD(P)H no activity with lipoic acid, cytochrome c and vitamin K1 Bos taurus K4[Fe(CN)6] + NAD(P)+
-
 ?
NAD(P)H + H+ + oxidized 2,6-dichlorophenolindophenol
-
 Rattus norvegicus NAD(P)+ + reduced 2,6-dichlorophenolindophenol
-
 ?
NAD(P)H + H+ + oxidized 2,6-dichlorophenolindophenol
-
 Bos taurus NAD(P)+ + reduced 2,6-dichlorophenolindophenol
-
 ?
vitamin K + NAD(P)H + H+
-
 Bos taurus reduced vitamin K + NAD(P)+
-
 ?
vitamin K2 + NAD(P)H
-
 Bos taurus reduced vitamin K2 + NAD(P)+
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
11700
-
 menadione
-
 Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.7
-
 menadione Bos taurus

pH Range

pH Minimum pH Maximum Comment Organism
4.8 9.8
-
 Bos taurus

Cofactor

Cofactor Comment Organism Structure
FAD enzyme contains FAD Rattus norvegicus
FAD enzyme contains FAD Bos taurus
FAD contains 1 mol of FAD per mol of enzyme Rattus norvegicus
FAD contains 1 mol of FAD per mol of enzyme Bos taurus
NADH
-
 Rattus norvegicus
NADH
-
 Bos taurus
NADPH
-
 Rattus norvegicus
NADPH
-
 Bos taurus