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Literature summary for 1.6.5.2 extracted from

  • Di Prisco, G.; Casola, L.; Giuditta, A.
    Purification and properties of a soluble reduced nicotinamide-adenine dinucleotide (phosphate) dehydrogenase from the hepatopancreas of Octopus vulgaris (1967), Biochem. J., 105, 455-460.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol stimulates Octopus vulgaris
cysteine stimulates Octopus vulgaris
GSH maximal stimulation with 0.5 mM, approx. 3fold activity Octopus vulgaris

Inhibitors

Inhibitors Comment Organism Structure
3,3'-methylene-bis(4-hydroxycoumarin) only in the presence of glutathione Octopus vulgaris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.034
-
NADH
-
Octopus vulgaris
0.22
-
NADPH
-
Octopus vulgaris

Organism

Organism UniProt Comment Textmining
Octopus vulgaris
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Octopus vulgaris

Source Tissue

Source Tissue Comment Organism Textmining
hepatopancreas
-
Octopus vulgaris
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8
-
-
Octopus vulgaris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzoquinone + 2 NAD(P)H + H+ no reaction with p-benzoquinone Octopus vulgaris benzohydroquinone + 2 NAD(P)+
-
?
ferricyanide + NADH + H+
-
Octopus vulgaris ferrocyanide + NAD+
-
r
K3[Fe(CN)6] + NAD(P)H no activity with O2, vitamin K3, ubiquinone-30 and 2-p-iodophenyl-3-p-nitrophenyl-5-phenyltetrazolium chloride Octopus vulgaris K4[Fe(CN)6] + NAD(P)+
-
?
K3[Fe(CN)6] + NAD(P)H no activity with lipoic acid, cytochrome c and vitamin K1 Octopus vulgaris K4[Fe(CN)6] + NAD(P)+
-
?
NAD(P)H + H+ + oxidized 2,6-dichlorophenolindophenol low activity Octopus vulgaris NAD(P)+ + reduced 2,6-dichlorophenolindophenol
-
?

Cofactor

Cofactor Comment Organism Structure
FMN contains FNN as prosthetic group Octopus vulgaris

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.058
-
dicoumarol cofactor NADH Octopus vulgaris
0.062
-
dicoumarol cofactor NADPH Octopus vulgaris