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Literature summary for 1.6.5.2 extracted from

  • Wosilait, W.D.
    The reduction of vitamin K1 by an enzyme from dog liver (1960), J. Biol. Chem., 235, 1196-1201.
    View publication on PubMed
Search for more literature for 1.6.5.2

General Stability

General Stability Organism
stable to dialysis against 0.01 M potassium phosphate buffer, pH 7.5 and 0.01 M Tris buffer, pH 8.2 and 8.9, 16 h or more at 3°C Canis lupus familiaris

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 1 mM, 50% inhibition Canis lupus familiaris
2,4-Dinitroaniline 0.1 mM, 33% inhibition Canis lupus familiaris
2,4-Dinitronaphthol 0.1 mM, 90% inhibition Canis lupus familiaris
2,4-dinitrophenol 0.1 mM, 39% inhibition Canis lupus familiaris
3,3'-methylene-bis(4-hydroxycoumarin) 0.1 mM, 97% inhibition Canis lupus familiaris
8-hydroxyquinoline 1 mM, 50% inhibition Canis lupus familiaris
alpha,alpha'-dipyridyl IC50: 1 mM Canis lupus familiaris
ethyl bis(2-hydroxy-4-oxo-4H-chromen-3-yl)acetate trivial tromexan, 0.1 mM, 79% inhibition Canis lupus familiaris
Phenindione 0.1 mM, 92% inhibition Canis lupus familiaris
salicylate 1 mM, 62% inhibition Canis lupus familiaris
warfarin 1 mM, 70% inhibition Canis lupus familiaris

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Canis lupus familiaris 5739
-

Organism

Organism UniProt Comment Textmining
Canis lupus familiaris
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Canis lupus familiaris

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Canis lupus familiaris
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.9
-
-
 Canis lupus familiaris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-methyl-1,4-naphthoquinone + NAD(P)H artificial acceptor, trivial name menadione Canis lupus familiaris 2-methyl-1,4-naphthoquinol + NAD(P)+
-
 ?
2-methyl-3-phytyl-1,4-naphthoquinone + NAD(P)H trivial name vitamin K1 Canis lupus familiaris 2-methyl-3-phytyl-1,4-naphthohydroquinone + NAD(P)+
-
 ?
2-methyl-3-phytyl-1,4-naphthoquinone + NAD(P)H no activity with vitamin K1 diphosphate, menadione diphosphate, vitamin K1 oxide, d-alpha-tocopherol and d-alpha-tocoquinone Canis lupus familiaris 2-methyl-3-phytyl-1,4-naphthohydroquinone + NAD(P)+
-
 ?
benzoquinone + 2 NAD(P)H + H+
-
 Canis lupus familiaris benzohydroquinone + 2 NAD(P)+
-
 ?
coenzyme Q10 + NAD(P)H
-
 Canis lupus familiaris reduced coenzyme Q10 + NAD(P)+
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
-
 some days Canis lupus familiaris
20
-
 several h Canis lupus familiaris
57
-
 5 min, 90% loss of activity Canis lupus familiaris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.7
-
 decrease above Canis lupus familiaris

Cofactor

Cofactor Comment Organism Structure
deamino-NADH
-
 Canis lupus familiaris
FAD stimulates Canis lupus familiaris
FMN stimulation Canis lupus familiaris
NADH
-
 Canis lupus familiaris
NADPH
-
 Canis lupus familiaris

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1
-
 IC50: 1 mM Canis lupus familiaris alpha,alpha'-dipyridyl