Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 Star (DE3) pLysS cells | Synechocystis sp. |
expressed in Escherichia coli BL21 Star (DE3) pLysS cells | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dicumarol | - |
Arabidopsis thaliana | |
dicumarol | - |
Synechocystis sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0024 | - |
menadione | pH 7.5, 30°C, recombinant enzyme | Synechocystis sp. | |
0.02 | - |
menadione | pH 7.5, 30°C, recombinant enzyme | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Arabidopsis thaliana | 9507 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
demethylphylloquinone + NADPH + H+ | Synechocystis sp. | - |
demethylphylloquinol + NADP+ | - |
? | |
demethylphylloquinone + NADPH + H+ | Arabidopsis thaliana | - |
demethylphylloquinol + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q8GXR9 | - |
- |
Synechocystis sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA His-Bind resin column chromatography | Synechocystis sp. |
Ni-NTA His-Bind resin column chromatography | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
demethylphylloquinone + NADPH + H+ | - |
Synechocystis sp. | demethylphylloquinol + NADP+ | - |
? | |
demethylphylloquinone + NADPH + H+ | - |
Arabidopsis thaliana | demethylphylloquinol + NADP+ | - |
? | |
menadione + NADPH + H+ | - |
Synechocystis sp. | menadiol + NADP+ | - |
? | |
menadione + NADPH + H+ | - |
Arabidopsis thaliana | menadiol + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NAD(P)H dehydrogenase C1 | - |
Arabidopsis thaliana |
ndbB | - |
Synechocystis sp. |
NDC1 | - |
Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.44 | - |
menadione | pH 7.5, 30°C, recombinant enzyme | Arabidopsis thaliana | |
3.2 | - |
menadione | pH 7.5, 30°C, recombinant enzyme | Synechocystis sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Synechocystis sp. | |
FAD | - |
Arabidopsis thaliana | |
NADPH | - |
Synechocystis sp. | |
NADPH | - |
Arabidopsis thaliana |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0013 | - |
dicumarol | substrate menadione and NADPH | Arabidopsis thaliana | |
0.0055 | - |
dicumarol | substrate menadione and NADPH | Synechocystis sp. |
General Information | Comment | Organism |
---|---|---|
malfunction | enzyme deletion mutants display increased photosensitivity to high light | Synechocystis sp. |
malfunction | enzyme deletion mutants display increased photosensitivity to high light | Arabidopsis thaliana |
metabolism | the enzyme performs the penultimate step in the biosynthesis of vitamin K1 | Synechocystis sp. |
metabolism | the enzyme performs the penultimate step in the biosynthesis of vitamin K1 | Arabidopsis thaliana |