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Literature summary for 1.6.5.12 extracted from

  • Fatihi, A.; Latimer, S.; Schmollinger, S.; Block, A.; Dussault, P.H.; Vermaas, W.F.; Merchant, S.S.; Basset, G.J.
    A dedicated type II NADPH dehydrogenase performs the penultimate step in the biosynthesis of vitamin K1 in Synechocystis and Arabidopsis (2015), Plant Cell, 27, 1730-1741.
    View publication on PubMed
Search for more literature for 1.6.5.12

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 Star (DE3) pLysS cells Arabidopsis thaliana
expressed in Escherichia coli BL21 Star (DE3) pLysS cells Synechocystis sp.

Inhibitors

Inhibitors Comment Organism Structure
dicumarol
-
 Arabidopsis thaliana
dicumarol
-
 Synechocystis sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0024
-
 menadione pH 7.5, 30°C, recombinant enzyme Synechocystis sp.
0.02
-
 menadione pH 7.5, 30°C, recombinant enzyme Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Arabidopsis thaliana 9507
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
demethylphylloquinone + NADPH + H+ Synechocystis sp.
-
 demethylphylloquinol + NADP+
-
 ?
demethylphylloquinone + NADPH + H+ Arabidopsis thaliana
-
 demethylphylloquinol + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q8GXR9
-
-
Synechocystis sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA His-Bind resin column chromatography Arabidopsis thaliana
Ni-NTA His-Bind resin column chromatography Synechocystis sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
demethylphylloquinone + NADPH + H+
-
 Synechocystis sp. demethylphylloquinol + NADP+
-
 ?
demethylphylloquinone + NADPH + H+
-
 Arabidopsis thaliana demethylphylloquinol + NADP+
-
 ?
menadione + NADPH + H+
-
 Synechocystis sp. menadiol + NADP+
-
 ?
menadione + NADPH + H+
-
 Arabidopsis thaliana menadiol + NADP+
-
 ?

Synonyms

Synonyms Comment Organism
NAD(P)H dehydrogenase C1
-
 Arabidopsis thaliana
ndbB
-
 Synechocystis sp.
NDC1
-
 Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.44
-
 menadione pH 7.5, 30°C, recombinant enzyme Arabidopsis thaliana
3.2
-
 menadione pH 7.5, 30°C, recombinant enzyme Synechocystis sp.

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Synechocystis sp.
FAD
-
 Arabidopsis thaliana
NADPH
-
 Synechocystis sp.
NADPH
-
 Arabidopsis thaliana

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0013
-
 dicumarol substrate menadione and NADPH Arabidopsis thaliana
0.0055
-
 dicumarol substrate menadione and NADPH Synechocystis sp.

General Information

General Information Comment Organism
malfunction enzyme deletion mutants display increased photosensitivity to high light Synechocystis sp.
malfunction enzyme deletion mutants display increased photosensitivity to high light Arabidopsis thaliana
metabolism the enzyme performs the penultimate step in the biosynthesis of vitamin K1 Synechocystis sp.
metabolism the enzyme performs the penultimate step in the biosynthesis of vitamin K1 Arabidopsis thaliana