BRENDA - Enzyme Database show
show all sequences of 1.6.5.10

The NADPH quinone reductase MdaB confers oxidative stress resistance to Helicobacter hepaticus

Hong, Y.; Wang, G.; Maier, R.J.; Microb. Pathog. 44, 169-174 (2008)

Data extracted from this reference:

Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Helicobacter hepaticus
-
strain ATCC51449
-
Helicobacter hepaticus ATCC 51449
-
strain ATCC51449
-
Purification (Commentary)
Commentary
Organism
-
Helicobacter hepaticus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,4-naphthoquinone + NADPH + H+
two-electron transfer mechanism from NADPH to quinone
688840
Helicobacter hepaticus
1,4-naphthoquinol + NADP+
-
-
-
?
2 ferricyanide + NADPH
-
688840
Helicobacter hepaticus
2 ferrocyanide + NADP+ + H+
-
-
-
?
menadione + NADPH + H+
two-electron transfer mechanism from NADPH to quinone
688840
Helicobacter hepaticus
? + NADP+
-
-
-
?
oxidized dichlorophenolindophenol + NADPH + H+
-
688840
Helicobacter hepaticus
reduced dicholorophenolindophenol + NADP+
-
-
-
?
ubiquinone-0 + NADPH + H+
two-electron transfer mechanism from NADPH to quinone
688840
Helicobacter hepaticus
ubiquinol-0 + NADP+
-
-
-
?
ubiquinone-1 + NADPH + H+
two-electron transfer mechanism from NADPH to quinone
688840
Helicobacter hepaticus
ubiquinol-1 + NADP+
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 21000-22000, SDS-PAGE
Helicobacter hepaticus
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
22
-
assay at room temperature
Helicobacter hepaticus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Helicobacter hepaticus
Cofactor
Cofactor
Commentary
Organism
Structure
flavin
flavoprotein, adsorption spectrum with a major peak at 456 nm
Helicobacter hepaticus
NADPH
NADH could also be used as an electron donor to MdaB but with much lower efficiency, about 3% of those with NADPH
Helicobacter hepaticus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
flavin
flavoprotein, adsorption spectrum with a major peak at 456 nm
Helicobacter hepaticus
NADPH
NADH could also be used as an electron donor to MdaB but with much lower efficiency, about 3% of those with NADPH
Helicobacter hepaticus
Purification (Commentary) (protein specific)
Commentary
Organism
-
Helicobacter hepaticus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,4-naphthoquinone + NADPH + H+
two-electron transfer mechanism from NADPH to quinone
688840
Helicobacter hepaticus
1,4-naphthoquinol + NADP+
-
-
-
?
2 ferricyanide + NADPH
-
688840
Helicobacter hepaticus
2 ferrocyanide + NADP+ + H+
-
-
-
?
menadione + NADPH + H+
two-electron transfer mechanism from NADPH to quinone
688840
Helicobacter hepaticus
? + NADP+
-
-
-
?
oxidized dichlorophenolindophenol + NADPH + H+
-
688840
Helicobacter hepaticus
reduced dicholorophenolindophenol + NADP+
-
-
-
?
ubiquinone-0 + NADPH + H+
two-electron transfer mechanism from NADPH to quinone
688840
Helicobacter hepaticus
ubiquinol-0 + NADP+
-
-
-
?
ubiquinone-1 + NADPH + H+
two-electron transfer mechanism from NADPH to quinone
688840
Helicobacter hepaticus
ubiquinol-1 + NADP+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 21000-22000, SDS-PAGE
Helicobacter hepaticus
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
22
-
assay at room temperature
Helicobacter hepaticus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Helicobacter hepaticus
Other publictions for EC 1.6.5.10
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743650
Brissos
Improving kinetic or thermody ...
Pseudomonas putida, Pseudomonas putida MET94
PLoS ONE
9
e87209
2014
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724333
Hervas
ArsH from the cyanobacterium S ...
Synechocystis sp.
Biochemistry
51
1178-1187
2012
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714708
Crosas
Novel alkenal/one reductase ac ...
Saccharomyces cerevisiae
Chem. Biol. Interact.
191
32-37
2011
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701035
Zai
Oxidative stress in tardive dy ...
Homo sapiens
Prog. Neuropsychopharmacol. Biol. Psychiatry
34
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688840
Hong
The NADPH quinone reductase Md ...
Helicobacter hepaticus, Helicobacter hepaticus ATCC 51449
Microb. Pathog.
44
169-174
2008
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690177
Huang
Arsenic methylation capability ...
Homo sapiens
Urol. Int.
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405-412
2008
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695605
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The natural compound ascorbige ...
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696015
Endo
Human carbonyl reductase 4 is ...
Homo sapiens
Biochem. Biophys. Res. Commun.
377
1326-1330
2008
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699129
Lambertucci
Palmitate increases superoxide ...
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699999
Laurindo
Assessment of superoxide produ ...
Homo sapiens
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441
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Gharavi
Role of nitric oxide in downre ...
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674461
Kitzing
The 1.3 A crystal structure of ...
Bacillus subtilis
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658899
Wang
An NADPH quinone reductase of ...
Helicobacter pylori
Infect. Immun.
72
1391-1396
2004
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655940
Kim
Molecular cloning and characte ...
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714140
Schlegel
Retention of NADPH-linked quin ...
Rattus norvegicus
Biochemistry
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1998
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Diatchuk
Inhibition of NADPH oxidase ac ...
Cavia porcellus, Homo sapiens
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714290
Hayashi
NADPH-specific quinone reducta ...
Escherichia coli
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1273
165-170
1996
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Batot
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Viljoen
-
Isolation and characterization ...
Microcystis aeruginosa
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392827
Pupillo
-
Pyridine nucleotide-linked deh ...
Cucurbita pepo, Zea mays
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321-328
1982
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Koli
Purification and properties of ...
Sus scrofa
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392782
Jagendorf
-
Chloroplast TPNH diaphorase ...
Spinacia oleracea
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430-434
1963
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