BRENDA - Enzyme Database show
show all sequences of 1.6.5.10

Molecular cloning and characterization of an NADPH quinone oxidoreductase from Kluyveromyces marxianus

Kim, W.H.; Chung, J.H.; Back, J.H.; Choi, J.; Cha, J.H.; Koh, H.Y.; Han, Y.S.; J. Biochem. Mol. Biol. 36, 442-449 (2003)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Kluyveromyces marxianus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.8
-
1,4-benzoquinone
25°C, pH 6.5
Kluyveromyces marxianus
298.2
-
2,3-dichloro-5,6-dicyano-1,4-benzoquinone
25°C, pH 6.5
Kluyveromyces marxianus
647.5
-
hydroquinone
25°C, pH 6.5
Kluyveromyces marxianus
728.6
-
phenyl-1,4-benzoquinone
25°C, pH 6.5
Kluyveromyces marxianus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
42000
-
2 * 42000, SDS-PAGE
Kluyveromyces marxianus
84000
-
gel filtration
Kluyveromyces marxianus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Kluyveromyces marxianus
Q8NJJ9
-
-
Purification (Commentary)
Commentary
Organism
-
Kluyveromyces marxianus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
NADPH + H+ + 1,4-benzoquinone
no activity with NADH
655940
Kluyveromyces marxianus
NADP+ + 1,4-benzohydroquinol
-
-
-
?
NADPH + H+ + 2,3-dichloro-5,6-dicyano-1,4-benzoquinone
-
655940
Kluyveromyces marxianus
NADP+ + 2,3-dichloro-5,6-dicyano-1,4-benzoquinol
-
-
-
?
NADPH + H+ + phenyl-1,4-benzoquinone
-
655940
Kluyveromyces marxianus
NADP+ + phenyl-1,4-benzoquinol
-
-
-
?
NADPH + H+ + quinone
-
655940
Kluyveromyces marxianus
NADP+ + quinol
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 42000, SDS-PAGE
Kluyveromyces marxianus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
360
-
phenyl-1,4-benzoquinone
25°C, pH 6.5
Kluyveromyces marxianus
520
-
2,3-dichloro-5,6-dicyano-1,4-benzoquinone
25°C, pH 6.5
Kluyveromyces marxianus
1600
-
hydroquinone
25°C, pH 6.5
Kluyveromyces marxianus
15833
-
1,4-benzoquinone
25°C, pH 6.5
Kluyveromyces marxianus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.1
-
calculation from nucleotide sequence
Kluyveromyces marxianus
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
no activity with NADH
Kluyveromyces marxianus
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Kluyveromyces marxianus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
no activity with NADH
Kluyveromyces marxianus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.8
-
1,4-benzoquinone
25°C, pH 6.5
Kluyveromyces marxianus
298.2
-
2,3-dichloro-5,6-dicyano-1,4-benzoquinone
25°C, pH 6.5
Kluyveromyces marxianus
647.5
-
hydroquinone
25°C, pH 6.5
Kluyveromyces marxianus
728.6
-
phenyl-1,4-benzoquinone
25°C, pH 6.5
Kluyveromyces marxianus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
42000
-
2 * 42000, SDS-PAGE
Kluyveromyces marxianus
84000
-
gel filtration
Kluyveromyces marxianus
Purification (Commentary) (protein specific)
Commentary
Organism
-
Kluyveromyces marxianus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
NADPH + H+ + 1,4-benzoquinone
no activity with NADH
655940
Kluyveromyces marxianus
NADP+ + 1,4-benzohydroquinol
-
-
-
?
NADPH + H+ + 2,3-dichloro-5,6-dicyano-1,4-benzoquinone
-
655940
Kluyveromyces marxianus
NADP+ + 2,3-dichloro-5,6-dicyano-1,4-benzoquinol
-
-
-
?
NADPH + H+ + phenyl-1,4-benzoquinone
-
655940
Kluyveromyces marxianus
NADP+ + phenyl-1,4-benzoquinol
-
-
-
?
NADPH + H+ + quinone
-
655940
Kluyveromyces marxianus
NADP+ + quinol
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 42000, SDS-PAGE
Kluyveromyces marxianus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
360
-
phenyl-1,4-benzoquinone
25°C, pH 6.5
Kluyveromyces marxianus
520
-
2,3-dichloro-5,6-dicyano-1,4-benzoquinone
25°C, pH 6.5
Kluyveromyces marxianus
1600
-
hydroquinone
25°C, pH 6.5
Kluyveromyces marxianus
15833
-
1,4-benzoquinone
25°C, pH 6.5
Kluyveromyces marxianus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.1
-
calculation from nucleotide sequence
Kluyveromyces marxianus
Other publictions for EC 1.6.5.10
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743650
Brissos
Improving kinetic or thermody ...
Pseudomonas putida, Pseudomonas putida MET94
PLoS ONE
9
e87209
2014
-
-
1
-
21
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-
1
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7
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12
1
1
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1
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1
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21
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1
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12
1
1
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1
-
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1
1
-
-
-
724333
Hervas
ArsH from the cyanobacterium S ...
Synechocystis sp.
Biochemistry
51
1178-1187
2012
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1
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2
9
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1
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1
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10
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7
1
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2
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9
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10
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1
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7
1
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4
4
-
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714708
Crosas
Novel alkenal/one reductase ac ...
Saccharomyces cerevisiae
Chem. Biol. Interact.
191
32-37
2011
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1
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2
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3
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-
2
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1
1
1
1
2
2
701035
Zai
Oxidative stress in tardive dy ...
Homo sapiens
Prog. Neuropsychopharmacol. Biol. Psychiatry
34
50-56
2009
-
1
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1
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1
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688840
Hong
The NADPH quinone reductase Md ...
Helicobacter hepaticus, Helicobacter hepaticus ATCC 51449
Microb. Pathog.
44
169-174
2008
-
-
-
-
-
-
-
-
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2
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1
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6
1
1
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1
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2
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2
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1
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6
1
1
-
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1
-
-
-
-
-
-
-
-
-
690177
Huang
Arsenic methylation capability ...
Homo sapiens
Urol. Int.
80
405-412
2008
-
1
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1
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1
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695605
Wagner
The natural compound ascorbige ...
Rattus norvegicus
Ann. Nutr. Metab.
53
122-128
2008
-
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4
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2
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1
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696015
Endo
Human carbonyl reductase 4 is ...
Homo sapiens
Biochem. Biophys. Res. Commun.
377
1326-1330
2008
-
-
1
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4
9
1
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2
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1
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4
-
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10
1
1
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-
7
1
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1
3
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1
1
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4
3
9
1
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2
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4
-
-
10
1
1
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-
7
1
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699129
Lambertucci
Palmitate increases superoxide ...
Rattus norvegicus
J. Cell. Physiol.
216
796-804
2008
1
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1
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2
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2
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1
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1
1
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-
-
-
699999
Laurindo
Assessment of superoxide produ ...
Homo sapiens
Methods Enzymol.
441
237-260
2008
-
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1
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3
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1
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1
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1
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1
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1
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3
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1
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2
1
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1
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1
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688593
Gharavi
Role of nitric oxide in downre ...
Mus musculus
J. Pharm. Sci.
96
2795-2807
2007
1
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2
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674461
Kitzing
The 1.3 A crystal structure of ...
Bacillus subtilis
J. Biol. Chem.
280
27904-27913
2005
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1
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2
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658899
Wang
An NADPH quinone reductase of ...
Helicobacter pylori
Infect. Immun.
72
1391-1396
2004
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1
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1
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13
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13
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655940
Kim
Molecular cloning and characte ...
Kluyveromyces marxianus
J. Biochem. Mol. Biol.
36
442-449
2003
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1
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4
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2
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3
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1
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1
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4
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1
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714140
Schlegel
Retention of NADPH-linked quin ...
Rattus norvegicus
Biochemistry
37
11003-11011
1998
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6
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6
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1
8
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4
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1
8
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1
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3
3
394277
Diatchuk
Inhibition of NADPH oxidase ac ...
Cavia porcellus, Homo sapiens
J. Biol. Chem.
272
13292-13301
1997
1
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714290
Hayashi
NADPH-specific quinone reducta ...
Escherichia coli
Biochim. Biophys. Acta
1273
165-170
1996
1
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2
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5
1
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1
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1
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5
1
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1
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394276
Batot
Characterization of neutrophil ...
Homo sapiens
Eur. J. Biochem.
234
208-215
1995
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1
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1
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1
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2
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1
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1
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394367
Viljoen
-
Isolation and characterization ...
Microcystis aeruginosa
Biochim. Biophys. Acta
827
247-259
1985
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6
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1
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3
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6
1
1
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1
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1
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1
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6
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1
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1
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1
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6
1
1
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1
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392827
Pupillo
-
Pyridine nucleotide-linked deh ...
Cucurbita pepo, Zea mays
Dev. Plant Biol.
7
321-328
1982
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1
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2
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2
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392826
Koli
Purification and properties of ...
Sus scrofa
J. Biol. Chem.
244
621-629
1969
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3
2
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1
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1
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1
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4
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1
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1
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1
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3
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2
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1
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1
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4
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1
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392782
Jagendorf
-
Chloroplast TPNH diaphorase ...
Spinacia oleracea
Methods Enzymol.
6
430-434
1963
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6
1
1
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1
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9
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2
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2
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6
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1
1
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1
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1
1
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9
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