Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Kluyveromyces marxianus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.8 | - |
1,4-benzoquinone | 25°C, pH 6.5 | Kluyveromyces marxianus | |
298.2 | - |
2,3-dichloro-5,6-dicyano-1,4-benzoquinone | 25°C, pH 6.5 | Kluyveromyces marxianus | |
647.5 | - |
hydroquinone | 25°C, pH 6.5 | Kluyveromyces marxianus | |
728.6 | - |
phenyl-1,4-benzoquinone | 25°C, pH 6.5 | Kluyveromyces marxianus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
2 * 42000, SDS-PAGE | Kluyveromyces marxianus |
84000 | - |
gel filtration | Kluyveromyces marxianus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Kluyveromyces marxianus | Q8NJJ9 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Kluyveromyces marxianus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADPH + H+ + 1,4-benzoquinone | no activity with NADH | Kluyveromyces marxianus | NADP+ + 1,4-benzohydroquinol | - |
? | |
NADPH + H+ + 2,3-dichloro-5,6-dicyano-1,4-benzoquinone | - |
Kluyveromyces marxianus | NADP+ + 2,3-dichloro-5,6-dicyano-1,4-benzoquinol | - |
? | |
NADPH + H+ + phenyl-1,4-benzoquinone | - |
Kluyveromyces marxianus | NADP+ + phenyl-1,4-benzoquinol | - |
? | |
NADPH + H+ + quinone | - |
Kluyveromyces marxianus | NADP+ + quinol | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 42000, SDS-PAGE | Kluyveromyces marxianus |
Synonyms | Comment | Organism |
---|---|---|
NADPH quinone oxidoreductase | - |
Kluyveromyces marxianus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
360 | - |
phenyl-1,4-benzoquinone | 25°C, pH 6.5 | Kluyveromyces marxianus | |
520 | - |
2,3-dichloro-5,6-dicyano-1,4-benzoquinone | 25°C, pH 6.5 | Kluyveromyces marxianus | |
1600 | - |
hydroquinone | 25°C, pH 6.5 | Kluyveromyces marxianus | |
15833 | - |
1,4-benzoquinone | 25°C, pH 6.5 | Kluyveromyces marxianus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.1 | - |
calculation from nucleotide sequence | Kluyveromyces marxianus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | no activity with NADH | Kluyveromyces marxianus |