Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 1 mM, 72% inhibition | Microcystis aeruginosa | |
1-(2-thenoyl)-3,3,3-trifluoracetone | 1 mM, 89% inhibition | Microcystis aeruginosa | |
2,4-dinitrophenol | 1 mM, 86% inhibition | Microcystis aeruginosa | |
8-hydroxyquinoline | 0.3 mM, 80% inhibition | Microcystis aeruginosa | |
ATP | 0.2 mM, 75% inhibition | Microcystis aeruginosa | |
EDTA | 0.15 mM, 80% inhibition | Microcystis aeruginosa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Microcystis aeruginosa | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
5100 | - |
8 * 5100, SDS-PAGE | Microcystis aeruginosa |
60260 | - |
enzyme form DII, gel filtration | Microcystis aeruginosa |
66070 | - |
enzyme form DI, gel filtration | Microcystis aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Microcystis aeruginosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Microcystis aeruginosa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
90300 units/mg, 1 unit is defined as that amount of enzyme causing an absorption change of 0.001 units/min at the specific wavelength | Microcystis aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,4-naphthoquinone + NADPH + H+ | specific for NADPH | Microcystis aeruginosa | 1,4-naphthoquinol + NADP+ | - |
? | |
2-methyl-1,4-naphthoquinone + NADPH + H+ | i.e. menadione, specific for NADPH | Microcystis aeruginosa | 2-methyl-1,4-naphthoquinol + NADP+ | - |
? | |
2-methyl-5-hydroxy-1,4-naphthoquinone + NADPH + H+ | - |
Microcystis aeruginosa | 2-methyl-5-hydroxy-1,4-naphthoquinol + NADP+ | - |
? | |
5-hydroxy-1,4-naphthoquinone + NADPH + H+ | - |
Microcystis aeruginosa | 5-hydroxy-1,4-naphthoquinol + NADP+ | - |
? | |
benzoquinone + NADPH | specific for NADPH | Microcystis aeruginosa | benzohydroquinone + NADP+ | - |
? | |
oxidized 2,6-dichlorophenolindophenol + NADPH + H+ | very low activity with NADH | Microcystis aeruginosa | reduced 2,6-dichlorophenolindophenol + NAD(P)+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | 8 * 5100, SDS-PAGE | Microcystis aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Microcystis aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | 9.5 | - |
Microcystis aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | very low activity with NADH | Microcystis aeruginosa |