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Literature summary for 1.6.3.3 extracted from

  • Masullo, M.; Raimo, G.; Dello Russo, A.; Bocchini, V.; Bannister, J.V.
    Purification and characterization of NADH oxidase from the archaea Sulfolobus acidocaldarius and Sulfolobus solfataricus (1996), Biotechnol. Appl. Biochem., 23, 47-54.
    View publication on PubMed
Search for more literature for 1.6.3.3

Inhibitors

Inhibitors Comment Organism Structure
Guanidine-HCl
-
 Saccharolobus solfataricus
Guanidine-HCl
-
 Sulfolobus acidocaldarius
Urea
-
 Saccharolobus solfataricus
Urea
-
 Sulfolobus acidocaldarius

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
 1 * 27000 Sulfolobus acidocaldarius
35000
-
 2 * 35000 Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NADH + H+ + O2 Sulfolobus acidocaldarius
-
 NAD+ + H2O2
-
 ?
NADH + H+ + O2 Saccharolobus solfataricus
-
 NAD+ + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-
Sulfolobus acidocaldarius
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sulfolobus acidocaldarius
-
 Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NADH + H+ + O2
-
 Sulfolobus acidocaldarius NAD+ + H2O2
-
 ?
NADH + H+ + O2
-
 Saccharolobus solfataricus NAD+ + H2O2
-
 ?
NADH + H+ + O2 oxidizes specifically beta-NADH in the presence of molecular oxygen Sulfolobus acidocaldarius NAD+ + H2O2
-
 ?
NADH + H+ + O2 oxidizes specifically beta-NADH in the presence of molecular oxygen Saccharolobus solfataricus NAD+ + H2O2
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 35000 Saccharolobus solfataricus
monomer 1 * 27000 Sulfolobus acidocaldarius

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
 half-life: 180 min Sulfolobus acidocaldarius
98
-
 half-life: 77 min Saccharolobus solfataricus

Cofactor

Cofactor Comment Organism Structure
FAD requires the presence of a flavin cofactor, showing a high specificity for FAD. The enzyme does not contain a flavin molecule Sulfolobus acidocaldarius
FAD requires the presence of a flavin cofactor, showing a high specificity for FAD. The enzyme is purified as an FAD-containing protein Saccharolobus solfataricus