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Literature summary for 1.6.3.3 extracted from

  • Case, C.L.; Rodriguez, J.R.; Mukhopadhyay, B.
    Characterization of an NADH oxidase of the flavin-dependent disulfide reductase family from Methanocaldococcus jannaschii (2009), Microbiology, 155, 69-79.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichi coli C41 Methanocaldococcus jannaschii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.9
-
O2 pH 7.0, 83°C Methanocaldococcus jannaschii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
x * 50000, SDS-PAGE Methanocaldococcus jannaschii
86000
-
gel filtration Methanocaldococcus jannaschii

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii Q58065
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Methanocaldococcus jannaschii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 NADH + H+ + O2 the enzyme produces both H2O and H2O2. 62% of NADH-derived reducing equivalents are recovered as H2O2 and the rest probably generates H2O. The NADPH oxidase activity is about 5% compared to the activity with NADH Methanocaldococcus jannaschii 2 NAD+ + 2 H2O
-
?
NADPH + H+ + O2 the enzyme produces both H2O and H2O2. 62% of NADH-derived reducing equivalents are recovered as H2O2 and the rest probably generates H2O. The NADPH oxidase activity is about 5% compared to the activity with NADH Methanocaldococcus jannaschii NAD+ + H2O2
-
?

Subunits

Subunits Comment Organism
? x * 50000, SDS-PAGE Methanocaldococcus jannaschii

Synonyms

Synonyms Comment Organism
MJ0649
-
Methanocaldococcus jannaschii
MJNox
-
Methanocaldococcus jannaschii

Cofactor

Cofactor Comment Organism Structure
FAD flavoprotein Methanocaldococcus jannaschii
NADH the NADPH oxidase activity is about 5% compared to the activity with NADH Methanocaldococcus jannaschii