Application | Comment | Organism |
---|---|---|
synthesis | usage of the enzyme for regeneration of both NADP+ and NAD+ in alcohol dehydrogenase-catalyzed enantioselective oxidation of racemic 1-phenylethanol. NADP+ regeneration at 30°C by TkNOX coupled with (R)-specific ADH from Lactobacillus kefir results in successful acquisition of optically pure (S)-1-phenylethanol, or at 45-60°C with moderately thermostable (S)-specific ADH from Rhodococcus erythropolis in optically pure (R)-1-phenylethanol, giving the possibility to operate the enantioselective bioconversion accompanying NAD+ regeneration at high temperatures, advantage of the combination of thermostable enzymes | Thermococcus kodakarensis |
Cloned (Comment) | Organism |
---|---|
gene tk0304, functional expression in Escherichia coli strain BL21-CodonPlus(DE3)-RIL | Thermococcus kodakarensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Thermococcus kodakarensis | KOD1 (TkNOX) catalyzes oxidation of NADH and NADPH with oxygen from atmospheric air as an electron acceptor | ? | - |
? | |
NADH + H+ + O2 | Thermococcus kodakarensis | - |
NAD+ + H2O2 | - |
? | |
NADPH + H+ + O2 | Thermococcus kodakarensis | - |
NADP+ + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus kodakarensis | Q5JFZ8 | gene tk0304 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain BL21-CodonPlus(DE3)-RIL partially by heat treatment at 65°C for 15 min | Thermococcus kodakarensis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.16 | - |
purified recombinant enzyme, with NADPH in a coupled reaction with (S)-specific ADH from Rhodococcus erythropolis, pH 7.0, 30°C | Thermococcus kodakarensis |
0.38 | - |
purified recombinant enzyme, with NADH in a coupled reaction with (S)-specific ADH from Rhodococcus erythropolis, pH 7.0, 30°C | Thermococcus kodakarensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | KOD1 (TkNOX) catalyzes oxidation of NADH and NADPH with oxygen from atmospheric air as an electron acceptor | Thermococcus kodakarensis | ? | - |
? | |
NADH + H+ + O2 | - |
Thermococcus kodakarensis | NAD+ + H2O2 | - |
? | |
NADPH + H+ + O2 | - |
Thermococcus kodakarensis | NADP+ + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
KOD1 | - |
Thermococcus kodakarensis |
NOX | - |
Thermococcus kodakarensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
above | Thermococcus kodakarensis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 90 | and above, activity range, profile overview | Thermococcus kodakarensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
the enzyme shows a 25% loss of activity after incubation at 65°C for 1 h | Thermococcus kodakarensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Thermococcus kodakarensis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
the partially purified ReADH shows a preference for an alkaline pH range for the oxidation of (RS)-1-phenylethanol in a coupled reaction with (S)-specific ADH from Rhodococcus erythropolis, the highest activity occurs at pH 12.0 | Thermococcus kodakarensis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Thermococcus kodakarensis | |
NADH | - |
Thermococcus kodakarensis | |
NADPH | - |
Thermococcus kodakarensis |