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Literature summary for 1.6.3.1 extracted from

  • Chen, J.; He, R.; Minshall, R.D.; Dinauer, M.C.; Ye, R.D.
    Characterization of a mutation in the Phox homology domain of the NADPH oxidase component p40phox identifies a mechanism for negative regulation of superoxide production (2007), J. Biol. Chem., 282, 30273-30284.
    View publication on PubMed
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Activating Compound

Activating Compound Comment Organism Structure
formyl-Met-Leu-Phe stimulation. In addition, heterologous expression of subunit p40phox markedly enhances superoxide production in stimulated cells. Upon stimulation with phorbol 12-myristate 13-acetate or formyl-Met-Leu-Phe, p40phox translocates to plasma membrane in a p67phox- and p47phox-dependent manner Homo sapiens
phorbol 12-myristate 13-acetate stimulation. In addition, heterologous expression of subunit p40phox markedly enhances superoxide production in stimulated cells. Upon stimulation with phorbol 12-myristate 13-acetate or formyl-Met-Leu-Phe, p40phox translocates to plasma membrane in a p67phox- and p47phox-dependent manner Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
stable transfection of COS-7 cells of subunits gp91phox, p22phox, p67phox, p47phox, and also p40phox fused to the N-terminus of green fluorescent protein Homo sapiens

Protein Variants

Protein Variants Comment Organism
R57Q mutation in the phophatidylinositol 3-phosphate binding region of subunit p47phox. Mutation abrogates phophatidylinositol 3-phosphate binding and produces a dominant inhibitory effect on agonist-induced superoxide production and membrane translocation of subunits p47phox and p67phox. Mutant p40phox displayes increased association with actin and moesin and is found enriched in the Triton X-100-insoluble fraction along with p67phox and p47phox Homo sapiens
R57Q/D289A double mutant of subunit p40phox. Mutant fails to associate with subunits p67phox or p47phox in co-immunoprecipitation and Western blotting assays and abolishes the dominant inhibitory effect of mutant R57Q in phorbol 12-myristate 13-acetate- or formyl-Met-Leu-Phe-induced superoxide production Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm full-length subunit p40phox exhibits a cytoplasmic localization pattern in resting cells. Upon stimulation with phorbol 12-myristate 13-acetate or formyl-Met-Leu-Phe, p40phox translocates to plasma membrane in a p67phox- and p47phox-dependent manner Homo sapiens 5737
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plasma membrane full-length subunit p40phox exhibits a cytoplasmic localization pattern in resting cells. Upon stimulation with phorbol 12-myristate 13-acetate or formyl-Met-Leu-Phe, p40phox translocates to plasma membrane in a p67phox- and p47phox-dependent manner Homo sapiens 5886
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Organism

Organism UniProt Comment Textmining
Homo sapiens
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