Literature summary for 1.6.3.1 extracted from

Nakamura, Y.; Ogihara, S.; Ohtaki, S.
Activation by ATP of calcium-dependent NADPH-oxidase generating hydrogen peroxide in thyroid plasma membranes (1987), J. Biochem., 102, 1121-1132.

Search for more literature for 1.6.3.1

Activating Compound

Activating Compound	Comment	Organism	Structure
2,6-dichlorophenolindophenol	-	Sus scrofa
ATP	2fold activation	Sus scrofa
cytochrome c	-	Sus scrofa
menadione	-	Sus scrofa

Inhibitors

Inhibitors	Comment	Organism	Structure
Cd2+	-	Sus scrofa
Mn2+	-	Sus scrofa
additional information	inhibition by addition of hexokinase and glucose to remove ATP	Sus scrofa
p-chloromercuribenzoate	-	Sus scrofa
sodium deoxycholate	no activity at 5 mg/ml	Sus scrofa
Triton X-100	no activity at 2 mg/ml	Sus scrofa
Zn2+	-	Sus scrofa

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.023	-	NADPH	pH 7, 37°C, 1.1 mM Ca2+	Sus scrofa

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
apical plasma membrane	-	Sus scrofa	16324	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ba2+	very low activity	Sus scrofa
Ca2+	required for full activity	Sus scrofa
Ca2+	regulates enzyme activity	Sus scrofa
Ca2+	half maximal activity with 0.0001 mM Ca2+	Sus scrofa
Cd2+	inhibitory	Sus scrofa
additional information	Mg2+ has no effect	Sus scrofa
Sr2+	can replace for Ca2+	Sus scrofa
Zn2+	inhibitory	Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
NAD(P)H + H+ + O2	Sus scrofa	production of hydrogen peroxide in thyroid gland that is required for thyroid hormone synthesis	NAD(P)+ + H2O2	-	?

Organism

Organism	UniProt	Comment	Textmining
Sus scrofa	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
partial	Sus scrofa

Source Tissue

Source Tissue	Comment	Organism	Textmining
thyroid gland	-	Sus scrofa	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.0007	-	pH 7., 37°C, 1.1 mM Ca2+	Sus scrofa

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
NAD(P)H + H+ + O2	-	Sus scrofa	NAD(P)+ + H2O2	-	?
NAD(P)H + H+ + O2	production of hydrogen peroxide in thyroid gland that is required for thyroid hormone synthesis	Sus scrofa	NAD(P)+ + H2O2	-	?

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0002	-	p-chloromercuribenzoate	-	Sus scrofa

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)