Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetate | slight | Glycine max | |
iodoacetamide | - |
Glycine max | |
nicotinate | - |
Glycine max |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0095 | - |
ferrileghemoglobin | - |
Glycine max | |
0.0188 | - |
NADH | - |
Glycine max |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Glycine max | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54000 | - |
2 * 54000, SDS-PAGE | Glycine max |
83000 | - |
equilibrium ultracentrifugation | Glycine max |
100000 | - |
gel filtration | Glycine max |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NAD(P)H + ferrileghemoglobin | Glycine max | - |
NAD(P)+ + ferroleghemoglobin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
soybean | - |
Purification (Comment) | Organism |
---|---|
- |
Glycine max |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root nodule | - |
Glycine max | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.218 | - |
reduction of ferrileghemoglobin | Glycine max |
Storage Stability | Organism |
---|---|
0°C, 37 days, 96% activity retains | Glycine max |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ferricytochrome c + NAD(P)H | reduced at 53% of leghemoglobin rate | Glycine max | 2 ferrocytochrome c + NAD(P)+ + H+ | - |
? | |
NAD(P)H + ferrileghemoglobin | - |
Glycine max | NAD(P)+ + ferroleghemoglobin | - |
? | |
NAD(P)H + ferrileghemoglobin | 31% activity with NADPH | Glycine max | NAD(P)+ + ferroleghemoglobin | - |
? | |
NAD(P)H + ferrimyoglobin | - |
Glycine max | NAD(P)+ + ferromyoglobin | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 54000, SDS-PAGE | Glycine max |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
heat labile | Glycine max |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.2 | - |
decrease of activity from 100% at pH 5.2, to 30% at pH 6.7, no measurements below 5.2 | Glycine max |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Glycine max | |
NADPH | 31% of NADH activity | Glycine max |