Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | competitive inhibition | Alburnus tarichi | |
Cu2+ | competitive inhibition | Alburnus tarichi | |
Hg2+ | competitive inhibition | Alburnus tarichi | |
additional information | benzalkonium chloride and acriavine do not show any effect on enzyme activity | Alburnus tarichi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005208 | - |
NADPH | at pH 8.0 and 50°C | Alburnus tarichi | |
0.01282 | - |
ferricytochrome c | at pH 8.0 and 50°C | Alburnus tarichi |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Alburnus tarichi | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
As5+ | activates | Alburnus tarichi | |
Ca2+ | activates | Alburnus tarichi | |
Co2+ | activates | Alburnus tarichi | |
Mn2+ | activates | Alburnus tarichi | |
additional information | Pb2+ , Fe3+ , Ni2+ , Cd2+ , Zn2+ , and Tl+ do not have any effect on enzyme activity | Alburnus tarichi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alburnus tarichi | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-cellulose column chromatography | Alburnus tarichi |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Alburnus tarichi | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADPH + H+ + ferricytochrome c | - |
Alburnus tarichi | NADP+ + ferrocytochrome c | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 70000, SDS-PAGE | Alburnus tarichi |
Synonyms | Comment | Organism |
---|---|---|
CPR | - |
Alburnus tarichi |
cytochrome P450 reductase | - |
Alburnus tarichi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Alburnus tarichi |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
7.6 | - |
the enzyme remains stable at pH 7.6 after 24 h | Alburnus tarichi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Alburnus tarichi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Alburnus tarichi | |
FMN | - |
Alburnus tarichi |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00136 | - |
Hg2+ | at pH 8.0 and 50°C | Alburnus tarichi | |
0.0016 | - |
Ag+ | at pH 8.0 and 50°C | Alburnus tarichi | |
0.27979 | - |
Cu2+ | at pH 8.0 and 50°C | Alburnus tarichi |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0021936 | - |
at pH 8.0 and 50°C | Alburnus tarichi | Hg2+ | |
0.0029877 | - |
at pH 8.0 and 50°C | Alburnus tarichi | Ag+ | |
0.39766 | - |
at pH 8.0 and 50°C | Alburnus tarichi | Cu2+ |