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Literature summary for 1.6.2.4 extracted from

  • Lee, G.Y.; Kim, H.M.; Ma, S.H.; Park, S.H.; Joung, Y.H.; Yun, C.H.
    Heterologous expression and functional characterization of the NADPH-cytochrome P450 reductase from Capsicum annuum (2014), Plant Physiol. Biochem., 82, 116-122 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
isoform CPR1 is expressed in Escherichia coli Rosetta(DE3)pLysS cells Capsicum annuum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.081
-
ferricytochrome c isoform CPR1, at pH 7.6 and 25°C Capsicum annuum

Organism

Organism UniProt Comment Textmining
Capsicum annuum A0A0N6XHR4 cultivar Bukang
-
Capsicum annuum B5LAU2 cultivar Bukang
-

Purification (Commentary)

Purification (Comment) Organism
2',5'-ADP Sepharose column chromatography Capsicum annuum

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
Capsicum annuum
-
fruit
-
Capsicum annuum
-
leaf
-
Capsicum annuum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NADPH + H+ + doxorubicin
-
Capsicum annuum NADP+ + 7-deoxydoxorubicinone
-
?
NADPH + H+ + ferricyanide
-
Capsicum annuum NADP+ + ferrocyanide
-
?
NADPH + H+ + ferricytochrome b5
-
Capsicum annuum NADP+ + ferrocytochrome b5
-
?
NADPH + H+ + ferricytochrome c
-
Capsicum annuum NADP+ + ferrocytochrome c
-
?
NADPH + H+ + oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
-
Capsicum annuum NADP+ + reduced 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
-
?
NADPH + H+ + oxidized 5-cyano-2,3-ditolyl tetrazolium chloride
-
Capsicum annuum NADP+ + reduced 5-cyano-2,3-ditolyl tetrazolium chloride
-
?
NADPH + H+ + oxidized tetrazolium
-
Capsicum annuum NADP+ + reduced tetrazolium
-
?

Subunits

Subunits Comment Organism
? x * 76000, SDS-PAGE Capsicum annuum

Synonyms

Synonyms Comment Organism
CPR1 isoform Capsicum annuum
CPR2 isoform Capsicum annuum
NADPH-cytochrome P450 reductase
-
Capsicum annuum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
56
-
melting temperature Capsicum annuum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
15.7
-
oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide isoform CPR1, at pH 7.6 and 25°C Capsicum annuum
45.7
-
ferricytochrome c isoform CPR1, at pH 7.6 and 25°C Capsicum annuum
80
-
ferricyanide isoform CPR1, at pH 7.6 and 25°C Capsicum annuum

Cofactor

Cofactor Comment Organism Structure
FAD
-
Capsicum annuum
FMN
-
Capsicum annuum

Expression

Organism Comment Expression
Capsicum annuum at the red ripe stage, the expression level of isoform CPR1 is more than 6fold greater than that in leaves or flowers up