Literature summary for 1.6.2.4 extracted from

Simtchouk, S.; Eng, J.L.; Meints, C.E.; Makins, C.; Wolthers, K.R.
Kinetic analysis of cytochrome P450 reductase from Artemisia annua reveals accelerated rates of NADH-dependent flavin reduction (2013), FEBS J., 280, 6627-6642 .

Search for more literature for 1.6.2.4

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Artemisia annua

Inhibitors

Inhibitors	Comment	Organism	Structure
2',5'-ADP	-	Artemisia annua
NAD+	-	Artemisia annua
NADP+	-	Artemisia annua

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00087	-	ferricytochrome c	at pH 7.5 and 25°C	Artemisia annua
0.00122	-	NADPH	at pH 7.5 and 25°C	Artemisia annua
26.76	-	NADH	at pH 7.5 and 25°C	Artemisia annua

Organism

Organism	UniProt	Comment	Textmining
Artemisia annua	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
NADH + H+ + ferricytochrome c	very low activity with NADH	Artemisia annua	NAD+ + ferricytochrome c	-	?
NADPH + H+ + ferricytochrome c	-	Artemisia annua	NADP+ + ferrocytochrome c	-	?

Subunits

Subunits	Comment	Organism
?	x * 75000, SDS-PAGE	Artemisia annua
?	x * 73275, calculated from amino acid sequence	Artemisia annua

Synonyms

Synonyms	Comment	Organism
CPR	-	Artemisia annua
cytochrome P450 reductase	-	Artemisia annua

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
16.5	-	NADH	at pH 7.5 and 25°C	Artemisia annua
65.9	-	NADPH	at pH 7.5 and 25°C	Artemisia annua

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Artemisia annua
FMN	-	Artemisia annua
NADPH	the enzyme shows a strong preference (85100-fold) for NADPH over NADH	Artemisia annua

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.00026	-	2',5'-ADP	at pH 7.5 and 25°C	Artemisia annua
0.00159	-	NADP+	at pH 7.5 and 25°C	Artemisia annua
0.0269	-	NAD+	at pH 7.5 and 25°C	Artemisia annua

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.000615	-	NADH	at pH 7.5 and 25°C	Artemisia annua
0.0541	-	NADPH	at pH 7.5 and 25°C	Artemisia annua

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Release 2023.1 (January 2023)