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Literature summary for 1.6.2.4 extracted from

  • Mothersole, R.G.; Meints, C.E.; Louder, A.; Wolthers, K.R.
    Role of active site loop in coenzyme binding and flavin reduction in cytochrome P450 reductase (2016), Arch. Biochem. Biophys., 606, 111-119 .
    View publication on PubMed
Search for more literature for 1.6.2.4

Protein Variants

Protein Variants Comment Organism
A635G/R636S the mutations lead to a modest increase in cytochrome c reduction, which is linked to weaker coenzyme binding and faster interflavin electron transfer Homo sapiens
D634A the variant elicits a modest increase in coenzyme binding affinity coupled with a 36fold reduction in cytochrome c turnover and a 17fold decrease in the pre-steady state rate of flavin reduction Homo sapiens
D634N the variant elicits a modest increase in coenzyme binding affinity coupled with a 10fold reduction in cytochrome c turnover and a 3fold decrease in the pre-steady state rate of flavin reduction Homo sapiens
R636A the mutation leads to a modest increase in cytochrome c reduction, which is linked to weaker coenzyme binding and faster interflavin electron transfer Homo sapiens
R636S the mutation leads to a modest increase in cytochrome c reduction, which is linked to weaker coenzyme binding and faster interflavin electron transfer Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
2',5'-ADP
-
 Homo sapiens
NADP+
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00014
-
 NADPH mutant enzyme D634N, at pH 7.5 and 25°C Homo sapiens
0.00019
-
 NADPH mutant enzyme D634A, at pH 7.5 and 25°C Homo sapiens
0.0007
-
 NADPH wild type enzyme, at pH 7.5 and 25°C Homo sapiens
0.00106
-
 NADPH mutant enzyme A635G, at pH 7.5 and 25°C Homo sapiens
0.00106
-
 NADPH mutant enzyme R636S, at pH 7.5 and 25°C Homo sapiens
0.0016
-
 NADPH mutant enzyme R636A, at pH 7.5 and 25°C Homo sapiens
0.0018
-
 NADPH mutant enzyme A635G/R636S, at pH 7.5 and 25°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
 Homo sapiens 5783
-
membrane
-
 Homo sapiens 16020
-
nuclear envelope
-
 Homo sapiens 5635
-

Organism

Organism UniProt Comment Textmining
Homo sapiens P16435
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NADPH + H+ + oxidized cytochrome c
-
 Homo sapiens NADP+ + reduced cytochrome c
-
 ?

Subunits

Subunits Comment Organism
? x * 78000, calculated from amino acid sequence Homo sapiens

Synonyms

Synonyms Comment Organism
CPR
-
 Homo sapiens
cytochrome P450 reductase
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.56
-
 NADPH mutant enzyme D634A, at pH 7.5 and 25°C Homo sapiens
2 8 NADPH mutant enzyme R636A, at pH 7.5 and 25°C Homo sapiens
2.21
-
 NADPH mutant enzyme D634N, at pH 7.5 and 25°C Homo sapiens
20
-
 NADPH wild type enzyme, at pH 7.5 and 25°C Homo sapiens
23.4
-
 NADPH mutant enzyme A635G, at pH 7.5 and 25°C Homo sapiens
30.5
-
 NADPH mutant enzyme R636S, at pH 7.5 and 25°C Homo sapiens
38.6
-
 NADPH mutant enzyme A635G/R636S, at pH 7.5 and 25°C Homo sapiens

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Homo sapiens
FMN
-
 Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00037
-
 2',5'-ADP mutant enzyme D634N, at pH 7.5 and 25°C Homo sapiens
0.00041
-
 2',5'-ADP mutant enzyme D634A, at pH 7.5 and 25°C Homo sapiens
0.00043
-
 2',5'-ADP mutant enzyme A635G, at pH 7.5 and 25°C Homo sapiens
0.00043
-
 2',5'-ADP mutant enzyme R636S, at pH 7.5 and 25°C Homo sapiens
0.0006
-
 2',5'-ADP wild type enzyme, at pH 7.5 and 25°C Homo sapiens
0.00066
-
 NADP+ mutant enzyme D634N, at pH 7.5 and 25°C Homo sapiens
0.00072
-
 NADP+ mutant enzyme R636S, at pH 7.5 and 25°C Homo sapiens
0.00074
-
 NADP+ mutant enzyme D634A, at pH 7.5 and 25°C Homo sapiens
0.001
-
 NADP+ wild type enzyme, at pH 7.5 and 25°C Homo sapiens
0.001
-
 NADP+ mutant enzyme A635G, at pH 7.5 and 25°C Homo sapiens
0.00131
-
 2',5'-ADP mutant enzyme R636A, at pH 7.5 and 25°C Homo sapiens
0.00174
-
 NADP+ mutant enzyme R636A, at pH 7.5 and 25°C Homo sapiens
0.0027
-
 NADP+ mutant enzyme A635G/R636S, at pH 7.5 and 25°C Homo sapiens
0.0045
-
 2',5'-ADP mutant enzyme A635G/R636S, at pH 7.5 and 25°C Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2900
-
 NADPH mutant enzyme D634A, at pH 7.5 and 25°C Homo sapiens
15800
-
 NADPH mutant enzyme D634N, at pH 7.5 and 25°C Homo sapiens
17500
-
 NADPH mutant enzyme R636A, at pH 7.5 and 25°C Homo sapiens
22100
-
 NADPH mutant enzyme A635G, at pH 7.5 and 25°C Homo sapiens
22100
-
 NADPH mutant enzyme A635G/R636S, at pH 7.5 and 25°C Homo sapiens
22100
-
 NADPH mutant enzyme R636S, at pH 7.5 and 25°C Homo sapiens
28200
-
 NADPH wild type enzyme, at pH 7.5 and 25°C Homo sapiens