Cloned (Comment) | Organism |
---|---|
gene cpr, DNA and amino acid sequence determination and analysis, expression of the N-terminally truncated and His6-tagged enzyme, lacking the putative membrane anchor, in Escherichia coli strain BL21 (DE3) | Starmerella apicola |
Protein Variants | Comment | Organism |
---|---|---|
additional information | truncation f the N-terminal putative membrane anchor leads to increased activity in reducing cytochrome c as compared to the wild-type enzyme, overview | Starmerella apicola |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Starmerella apicola | |
0.0138 | - |
cytochrome c | pH 7.5, 25°C | Starmerella apicola |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
76068 | - |
x * 76068, sequence calculation | Starmerella apicola |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADPH + H+ + cytochrome c | Starmerella apicola | - |
NADP+ + reduced cytochrome c | - |
? | |
NADPH + H+ + cytochrome c | Starmerella apicola ATCC 96134 | - |
NADP+ + reduced cytochrome c | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Starmerella apicola | H2ER12 | gene cpr | - |
Starmerella apicola ATCC 96134 | H2ER12 | gene cpr | - |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally truncated and His6-tagged enzyme, lacking the putative membrane anchor, from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography and gel filtration to over 98% purity | Starmerella apicola |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADPH + H+ + cytochrome c | - |
Starmerella apicola | NADP+ + reduced cytochrome c | - |
? | |
NADPH + H+ + cytochrome c | - |
Starmerella apicola ATCC 96134 | NADP+ + reduced cytochrome c | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 76068, sequence calculation | Starmerella apicola |
Synonyms | Comment | Organism |
---|---|---|
CPR | - |
Starmerella apicola |
cytochrome P450-diflavin reductase | - |
Starmerella apicola |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Starmerella apicola |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
31.92 | - |
cytochrome c | pH 7.5, 25°C | Starmerella apicola |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Starmerella apicola |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Starmerella apicola | |
FMN | - |
Starmerella apicola | |
additional information | CPR is a diflavin protein containing the flavin cofactors FAD, accepting hydride ions from NADPH, and FMN, accepting single electrons from FAD and reducing the heme center of the P450 monooxygenase domain | Starmerella apicola | |
NADPH | - |
Starmerella apicola |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Starmerella apicola | sequence calculation | - |
6 |
General Information | Comment | Organism |
---|---|---|
additional information | CPR is a diflavin protein containing the flavin cofactors FAD, accepting hydride ions from NADPH, and FMN, accepting single electrons from FAD and reducing the heme center of the P450 monooxygenase domain | Starmerella apicola |