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Literature summary for 1.6.2.4 extracted from
- Clinical, structural and functional implications of mutations and polymorphisms in human NADPH P450 oxidoreductase (2007), Fundam. Clin. Pharmacol., 21, 399-410.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A115V | Vmax/Km for cytochrome c is 63% of wild-type value, Vmax/Km for NADPH is 41% of wild-type value | Homo sapiens |
| A287P | Vmax/Km for cytochrome c is 9% of wild-type value, Vmax/Km for NADPH is 16% of wild-type value | Homo sapiens |
| A503V | Vmax/Km for cytochrome c is 69% of wild-type value, Vmax/Km for NADPH is 86% of wild-type value | Homo sapiens |
| C569Y | Vmax/Km for cytochrome c is 6% of wild-type value, Vmax/Km for NADPH is 2% of wild-type value | Homo sapiens |
| F646del | Vmax/Km for cytochrome c is 36% of wild-type value, Vmax/Km for NADPH is 94% of wild-type value | Homo sapiens |
| G413S | Vmax/Km for cytochrome c is 76% of wild-type value, Vmax/Km for NADPH is identical to wild-type value | Homo sapiens |
| G504R | Vmax/Km for cytochrome c is 53% of wild-type value, Vmax/Km for NADPH is 47% of wild-type value | Homo sapiens |
| G539R | Vmax/Km for cytochrome c is 9% of wild-type value, Vmax/Km for NADPH is 0.2% of wild-type value | Homo sapiens |
| L565P | Vmax/Km for cytochrome c is 14% of wild-type value, Vmax/Km for NADPH is 1.4% of wild-type value | Homo sapiens |
| M263V | Vmax/Km for cytochrome c is 76% of wild-type value, Vmax/Km for NADPH is 57% of wild-type value | Homo sapiens |
| P228L | Vmax/Km for cytochrome c is 75% of wild-type value, Vmax/Km for NADPH is 72% of wild-type value | Homo sapiens |
| Q153R | Vmax/Km for cytochrome c is 9% of wild-type value, Vmax/Km for NADPH is 11% of wild-type value | Homo sapiens |
| R316W | Vmax/Km for cytochrome c is 61% of wild-type value, Vmax/Km for NADPH is 77% of wild-type value | Homo sapiens |
| R457H | Vmax/Km for cytochrome c is 0.7% of wild-type value | Homo sapiens |
| T142A | Vmax/Km for cytochrome c is 49% of wild-type value, Vmax/Km for NADPH is 52% of wild-type value | Homo sapiens |
| V492E | Vmax/Km for cytochrome c is 0.3% of wild-type value | Homo sapiens |
| V608F | Vmax/Km for cytochrome c is 8% of wild-type value, Vmax/Km for NADPH is 3% of wild-type value | Homo sapiens |
| V631I | Vmax/Km for cytochrome c is 74% of wild-type value, Vmax/Km for NADPH is 23% of wild-type value | Homo sapiens |
| Y459H | Vmax/Km for cytochrome c is 0.4% of wild-type value | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 ferricytochrome c + NADPH | - |
Homo sapiens | 2 ferrocytochrome c + NADP+ + H+ | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NADPH P450 oxidoreductase | - |
Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.43 | - |
ferricytochrome c | mutant enzyme Y459H | Homo sapiens | |
| 0.47 | - |
ferricytochrome c | mutant enzyme V492E | Homo sapiens | |
| 0.5 | - |
ferricytochrome c | mutant enzyme R457H | Homo sapiens | |
| 0.67 | - |
NADPH | mutant enzyme G539R | Homo sapiens | |
| 0.87 | - |
NADPH | mutant enzyme C569Y | Homo sapiens | |
| 1.5 | - |
ferricytochrome c | mutant enzyme Q153R | Homo sapiens | |
| 1.7 | - |
NADPH | mutant enzyme L565P | Homo sapiens | |
| 1.73 | - |
ferricytochrome c | mutant enzyme C569Y | Homo sapiens | |
| 1.87 | - |
ferricytochrome c | mutant enzyme L565P | Homo sapiens | |
| 1.87 | - |
ferricytochrome c | mutant enzyme V608F | Homo sapiens | |
| 2.3 | - |
NADPH | mutant enzyme V608F | Homo sapiens | |
| 2.3 | - |
NADPH | mutant enzyme V631I | Homo sapiens | |
| 2.5 | - |
NADPH | mutant enzyme A287P | Homo sapiens | |
| 2.6 | - |
NADPH | mutant enzyme A115V | Homo sapiens | |
| 2.6 | - |
ferricytochrome c | mutant enzyme A287P | Homo sapiens | |
| 2.7 | - |
ferricytochrome c | mutant enzyme G539R | Homo sapiens | |
| 2.97 | - |
NADPH | mutant enzyme T142A | Homo sapiens | |
| 3.1 | - |
NADPH | mutant enzyme Q153R | Homo sapiens | |
| 3.6 | - |
ferricytochrome c | mutant enzyme T142A | Homo sapiens | |
| 3.63 | - |
NADPH | mutant enzyme G504R | Homo sapiens | |
| 3.8 | - |
NADPH | mutant enzyme P228L | Homo sapiens | |
| 3.97 | - |
NADPH | mutant enzyme A503V | Homo sapiens | |
| 4.3 | - |
NADPH | mutant enzyme F646del | Homo sapiens | |
| 4.4 | - |
NADPH | mutant enzyme G413S | Homo sapiens | |
| 4.6 | - |
ferricytochrome c | mutant enzyme G413S | Homo sapiens | |
| 4.87 | - |
NADPH | wild-type enzyme | Homo sapiens | |
| 4.9 | - |
NADPH | mutant enzyme M263V | Homo sapiens | |
| 4.9 | - |
ferricytochrome c | mutant enzyme P228L | Homo sapiens | |
| 4.9 | - |
NADPH | mutant enzyme R316W | Homo sapiens | |
| 5.2 | - |
ferricytochrome c | mutant enzyme G504R | Homo sapiens | |
| 5.4 | - |
ferricytochrome c | mutant enzyme R316W | Homo sapiens | |
| 5.47 | - |
ferricytochrome c | mutant enzyme V631I | Homo sapiens | |
| 5.73 | - |
ferricytochrome c | mutant enzyme A503V | Homo sapiens | |
| 5.8 | - |
ferricytochrome c | mutant enzyme A115V | Homo sapiens | |
| 6.4 | - |
ferricytochrome c | mutant enzyme F646del | Homo sapiens | |
| 6.47 | - |
ferricytochrome c | wild-type enzyme | Homo sapiens | |
| 6.7 | - |
ferricytochrome c | mutant enzyme M263V | Homo sapiens | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Homo sapiens | |
| FMN | - |
Homo sapiens | |
| NADPH | - |
Homo sapiens | |
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