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Literature summary for 1.6.2.4 extracted from

  • Warrilow, A.G.; Lamb, D.C.; Kelly, D.E.; Kelly, S.L.
    Phanerochaete chrysosporium NADPH-cytochrome P450 reductase kinetic mechanism (2002), Biochem. Biophys. Res. Commun., 299, 189-195.
    View publication on PubMed
Search for more literature for 1.6.2.4

Cloned(Commentary)

Cloned (Comment) Organism
native and truncated form expressed in Escherichia coli BL21(DE3)pLysS as His tag fusion protein Phanerodontia chrysosporium

Protein Variants

Protein Variants Comment Organism
DELTA1-23 truncated enzyme Phanerodontia chrysosporium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0019
-
 NADPH full length enzyme, pH 7.8, 25°C Phanerodontia chrysosporium
0.0022
-
 NADPH truncated enzyme, pH 7.8, 25°C Phanerodontia chrysosporium
0.011
-
 ferricytochrome c full length enzyme, pH 7.8, 25°C Phanerodontia chrysosporium
0.012
-
 ferricytochrome c truncated enzyme, pH 7.8, 25°C Phanerodontia chrysosporium

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Phanerodontia chrysosporium 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
79300
-
 theoretical mass, truncated enzyme Phanerodontia chrysosporium
81600
-
 theoretical mass, full length enzyme Phanerodontia chrysosporium
85900
-
 SDS-PAGE, truncated enzyme Phanerodontia chrysosporium
88200
-
 SDS-PAGE, full length enzyme Phanerodontia chrysosporium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 ferricytochrome P450 + NADPH Phanerodontia chrysosporium transfer of electrons during the CYP catalytic cycle 2 ferrocytochrome P450 + NADP+ + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Phanerodontia chrysosporium
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzymes using His-tag Phanerodontia chrysosporium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.27
-
 purified full length enzyme, pH 7.8, 25°C Phanerodontia chrysosporium
14.76
-
 purified truncated enzyme, pH 7.8, 25°C Phanerodontia chrysosporium

Storage Stability

Storage Stability Organism
-80°C, 20 mM phosphate buffer, pH 7.5, 20% glycerol Phanerodontia chrysosporium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ferricytochrome c + NADPH
-
 Phanerodontia chrysosporium 2 ferrocytochrome c + NADP+ + H+
-
 ?
2 ferricytochrome P450 + NADPH transfer of electrons during the CYP catalytic cycle Phanerodontia chrysosporium 2 ferrocytochrome P450 + NADP+ + H+
-
 ?

Synonyms

Synonyms Comment Organism
NADPH-cytochrome P450 reductase
-
 Phanerodontia chrysosporium
NADPH-ferrihemoprotein reductase
-
 Phanerodontia chrysosporium
NADPH:ferrihemoprotein oxidoreductase
-
 Phanerodontia chrysosporium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
19
-
 ferricytochrome c full length enzyme, pH 7.8, 25°C Phanerodontia chrysosporium
42
-
 ferricytochrome c truncated enzyme, pH 7.8, 25°C Phanerodontia chrysosporium

Cofactor

Cofactor Comment Organism Structure
FAD together with FMN Phanerodontia chrysosporium
FMN together with FAD Phanerodontia chrysosporium
NADPH
-
 Phanerodontia chrysosporium