Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | storage of the enzyme at -75°C for 4 months results in a slight activation of the enzyme activity | Petunia x hybrida | |
potassium phosphate | activity slightly higher than with sodium chloride or sodium sulfate | Petunia x hybrida |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | - |
Petunia x hybrida | 5783 | - |
microsome | - |
Petunia x hybrida | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
84000 | - |
Western blot analysis of fresh microsomal extracts | Petunia x hybrida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Petunia x hybrida | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using column chromatography on adenosine 2',5'-biphosphate-Sepharose, followed by high-performance anion-exchange chromatography | Petunia x hybrida |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | buds and nascent, open flowers | Petunia x hybrida | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.02 | 0.12 | flower buds, open flowers exhibits a similar value | Petunia x hybrida |
60 | 120 | purified enzyme | Petunia x hybrida |
Storage Stability | Organism |
---|---|
-75°C, months, 5 mM 2'-AMP | Petunia x hybrida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ferricytochrome c + NADPH | additional electron acceptor: neotetrazolium chloride | Petunia x hybrida | 2 ferrocytochrome c + NADP+ + H+ | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
- |
Petunia x hybrida |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | - |
Petunia x hybrida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Petunia x hybrida | |
FMN | - |
Petunia x hybrida | |
NADH | activity not detected | Petunia x hybrida | |
NADPH | - |
Petunia x hybrida |