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Literature summary for 1.6.2.4 extracted from

  • Omura, T.; Takesue, S.
    A new method for simultaneous purification of cytochrome b5 and NADPH-cytochrome c reductase from rat liver microsomes (1970), J. Biochem., 67, 249-257.
    View publication on PubMed

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Rattus norvegicus
-
-

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
Sprague-Dawley strain
-
Rattus norvegicus Sprague-Dawley
-
Sprague-Dawley strain
-

Purification (Commentary)

Purification (Comment) Organism
trypsin digestion followed by Sephadex filtration and column chromatography on DEAE-cellulose Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.025
-
electron donor: NADH, electron acceptor: dichlorophenolindophenol Rattus norvegicus
0.03
-
electron donor: NADPH, electron acceptor: cytochrome b5 Rattus norvegicus
0.055
-
electron donor: NADH, electron acceptor: cytochrome c Rattus norvegicus
17.9
-
electron donor: NADPH, electron acceptor: dichlorophenolindophenol Rattus norvegicus
37.6
-
purified enzyme Rattus norvegicus
40.5
-
electron donor: NADPH, electron acceptor: ferricyanide Rattus norvegicus
41.2
-
electron donor: NADPH, electron acceptor: cytochrome c Rattus norvegicus

Storage Stability

Storage Stability Organism
-15°C or -20°C, 10 mM phosphate buffer, pH 7.5, several months Rattus norvegicus
0°C, some days Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ferricytochrome c + NADPH additional electron acceptor: ferricyanide Rattus norvegicus 2 ferrocytochrome c + NADP+ + H+
-
?
2 ferricytochrome c + NADPH additional electron acceptor: cytochrome b5 Rattus norvegicus 2 ferrocytochrome c + NADP+ + H+
-
?
2 ferricytochrome c + NADPH additional electron acceptor: 2,6-dichlorophenolindophenol Rattus norvegicus 2 ferrocytochrome c + NADP+ + H+
-
?
2 ferricytochrome c + NADPH additional electron acceptor: ferricyanide Rattus norvegicus Sprague-Dawley 2 ferrocytochrome c + NADP+ + H+
-
?
2 ferricytochrome c + NADPH additional electron acceptor: cytochrome b5 Rattus norvegicus Sprague-Dawley 2 ferrocytochrome c + NADP+ + H+
-
?
2 ferricytochrome c + NADPH additional electron acceptor: 2,6-dichlorophenolindophenol Rattus norvegicus Sprague-Dawley 2 ferrocytochrome c + NADP+ + H+
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25 30 diluted solutions: gradual loss of activity Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
FAD
-
Rattus norvegicus
NADH less effective than NADPH Rattus norvegicus
NADPH
-
Rattus norvegicus