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Literature summary for 1.6.2.4 extracted from

  • Bertrand, J.C.; Gilewicz, M.; Bazin, H.; Azoulay, E.
    Purified detergent-solubilized NADPH-cytochrome c (P-450) reductase from Candida tropicalis grown on alkanes (1980), Biochem. Biophys. Res. Commun., 94, 889-893.
    View publication on PubMed
Search for more literature for 1.6.2.4

General Stability

General Stability Organism
FAD, FMN necessary for stabilization during purification Candida tropicalis

Inhibitors

Inhibitors Comment Organism Structure
HgCl2
-
 Candida tropicalis
p-chloromercuribenzoate
-
 Candida tropicalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 differences in MW partially due to method of solubilization Candida tropicalis
76000
-
 x * 76000, SDS-PAGE Candida tropicalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NADPH + ferricytochrome P450 Candida tropicalis part of the microsomal electron-transport chains and responsible for the transfer of reducing equivalents between NADPH or NADH and cytochrome P-450 ferrocytochrome P450 + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Candida tropicalis
-
 grown on alkanes or glycerol
-

Purification (Commentary)

Purification (Comment) Organism
column chromatography on phenyl-sepharose CL-4B, DEAE cellulose, hydroxylapatite and Sephadex G-150 Candida tropicalis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
19
-
 electron acceptor: neotetrazolium chloride Candida tropicalis
26
-
 electron acceptor: ferricyanide Candida tropicalis
28
-
 electron acceptor: menadione Candida tropicalis
42
-
 electron acceptor: dichlorophenolindophenol Candida tropicalis
56
-
-
 Candida tropicalis

Storage Stability

Storage Stability Organism
-70°C, 0.15 mM potassium phosphate buffer, pH 7, 1 mM mercaptoethanol, 1 mM EDTA, 1 micromol FMN, 1 micromol FAD, 0.3% Mulgofen BC-720, 30% glycerol, several months Candida tropicalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ferricytochrome c + NADPH additional electron acceptor: ferricyanide Candida tropicalis 2 ferrocytochrome c + NADP+ + H+
-
 ?
2 ferricytochrome c + NADPH additional electron acceptor: neotetrazolium chloride Candida tropicalis 2 ferrocytochrome c + NADP+ + H+
-
 ?
2 ferricytochrome c + NADPH additional electron acceptor: menadione Candida tropicalis 2 ferrocytochrome c + NADP+ + H+
-
 ?
2 ferricytochrome c + NADPH additional electron acceptor: cytochrome P450 Candida tropicalis 2 ferrocytochrome c + NADP+ + H+
-
 ?
2 ferricytochrome c + NADPH additional electron acceptor: 2,6-dichlorophenolindophenol Candida tropicalis 2 ferrocytochrome c + NADP+ + H+
-
 ?
NADPH + ferricytochrome P450 part of the microsomal electron-transport chains and responsible for the transfer of reducing equivalents between NADPH or NADH and cytochrome P-450 Candida tropicalis ferrocytochrome P450 + NADP+
-
 ?

Subunits

Subunits Comment Organism
? x * 76000, SDS-PAGE Candida tropicalis
More differences in subunit weight partially due to method of solubilization Candida tropicalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
 Candida tropicalis

Cofactor

Cofactor Comment Organism Structure
FAD tightly bound Candida tropicalis
FAD ratio FAD : FMN 1 : 1 Candida tropicalis
FMN tightly bound Candida tropicalis
FMN 1 mol per mol of enzyme Candida tropicalis
FMN ratio FAD : FMN 1 : 1 Candida tropicalis
NADPH
-
 Candida tropicalis