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Literature summary for 1.6.2.4 extracted from
- A cytosolic FAD-containing enzyme catalyzing cytochrome c reduction in Trypanosoma cruzi. I. Purification and some properties (1985), Arch. Biochem. Biophys., 239, 18-28.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Phenobarbital | stimulation | Trypanosoma cruzi |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Trypanosoma cruzi | |
| 0.00303 | - |
NADPH | - |
Trypanosoma cruzi | |
| 0.06289 | - |
ferricytochrome c | - |
Trypanosoma cruzi | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Trypanosoma cruzi | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 52000 | - |
2 * 52000, SDS-PAGE | Trypanosoma cruzi |
| 100000 | - |
gel filtration | Trypanosoma cruzi |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trypanosoma cruzi | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-cellulose and 2',5'-ADP-agarose column chromatography | Trypanosoma cruzi |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| epimastigote | - |
Trypanosoma cruzi | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.05 | - |
cholate and Lubrol Px | Trypanosoma cruzi |
| 0.059 | - |
no detergent | Trypanosoma cruzi |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -90°C or -20°C, 5-10% loss of activity, reactivation by FAD, 24 h | Trypanosoma cruzi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 ferricytochrome c + NADPH | additional electron acceptor: ferricyanide | Trypanosoma cruzi | 2 ferrocytochrome c + NADP+ + H+ | - |
? | |
| 2 ferricytochrome c + NADPH | additional electron acceptor: menadione | Trypanosoma cruzi | 2 ferrocytochrome c + NADP+ + H+ | - |
? | |
| 2 ferricytochrome c + NADPH | additional electron acceptor: 2,6-dichlorophenolindophenol | Trypanosoma cruzi | 2 ferrocytochrome c + NADP+ + H+ | - |
? | |
| ferricyanide + NADPH + H+ | - |
Trypanosoma cruzi | ferrocyanide + NADP+ | - |
r | |
| ferricytochrome c + NADPH + H+ | - |
Trypanosoma cruzi | ferrocytochrome c + NADP+ | - |
r | |
| NADPH + H+ + menadione | - |
Trypanosoma cruzi | NADP+ + menadiol | - |
r | |
| NADPH + H+ oxidized 2,6-dichlorophenolindophenol | - |
Trypanosoma cruzi | ferrocytochrome c + reduced 2,6-dichlorophenolindophenol | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 52000, SDS-PAGE | Trypanosoma cruzi |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| -90 | - |
inactivation | Trypanosoma cruzi |
| 2 | 4 | 1 day, inactivation | Trypanosoma cruzi |
| 20 | - |
1 day, from 5% to 10% loss of activity | Trypanosoma cruzi |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.45 | - |
menadione | - |
Trypanosoma cruzi | |
| 7.63 | - |
oxidized 2,6-dichlorophenolindophenol | - |
Trypanosoma cruzi | |
| 15 | - |
ferricytochrome c | - |
Trypanosoma cruzi | |
| 64.5 | - |
ferricyanide | - |
Trypanosoma cruzi | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | 1 mol per subunit | Trypanosoma cruzi | |
| NADPH | - |
Trypanosoma cruzi | |
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