Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | low ionic strength, slight stimulation | Helianthus tuberosus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | high ionic strength | Helianthus tuberosus | |
NADP+ | non-competitive inhibition | Helianthus tuberosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | cosubstrate NADH, biphasic kinetic | Helianthus tuberosus | |
0.0182 | - |
ferricytochrome c | determined on the basis of a sequential mechanism | Helianthus tuberosus | |
0.02 | - |
NADPH | - |
Helianthus tuberosus | |
0.0308 | - |
NADPH | determined on the basis of a sequential mechanism | Helianthus tuberosus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Helianthus tuberosus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | stimulation by increasing ionic strength | Helianthus tuberosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
differences in MW partially due to method of solubilization | Helianthus tuberosus |
82000 | - |
x * 82000, SDS-PAGE | Helianthus tuberosus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADPH + ferricytochrome P450 | Helianthus tuberosus | part of the microsomal electron-transport chains and responsible for the transfer of reducing equivalents between NADPH or NADH and cytochrome P-450 | ferrocytochrome P450 + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helianthus tuberosus | - |
L. var. Blanc commun., Jerusalem artichoke | - |
Purification (Comment) | Organism |
---|---|
anion exchange chromatography and affinity chromatography | Helianthus tuberosus |
Renatured (Comment) | Organism |
---|---|
reconstitution of monooxygenase system | Helianthus tuberosus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
tuber | - |
Helianthus tuberosus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
33 | - |
- |
Helianthus tuberosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ferricytochrome c + NADPH | additional electron acceptor: ferricyanide | Helianthus tuberosus | 2 ferrocytochrome c + NADP+ + H+ | - |
? | |
2 ferricytochrome c + NADPH | additional electron acceptor: cytochrome P450 | Helianthus tuberosus | 2 ferrocytochrome c + NADP+ + H+ | - |
? | |
ferricytochrome c + NADPH + H+ | - |
Helianthus tuberosus | ferrocytochrome c + NADP+ | - |
r | |
additional information | hydroxylation of coumarate | Helianthus tuberosus | ? | - |
? | |
NADPH + ferricytochrome P450 | part of the microsomal electron-transport chains and responsible for the transfer of reducing equivalents between NADPH or NADH and cytochrome P-450 | Helianthus tuberosus | ferrocytochrome P450 + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 82000, SDS-PAGE | Helianthus tuberosus |
More | differences in subunit weight partially due to method of solubilization | Helianthus tuberosus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | ratio FAD : FMN 1 : 1 | Helianthus tuberosus | |
FMN | ratio FAD : FMN 1 : 1 | Helianthus tuberosus | |
FMN | not implicated in plant NADPH-ferricyanide reductase | Helianthus tuberosus | |
NADH | low affinity | Helianthus tuberosus | |
NADPH | - |
Helianthus tuberosus |