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Literature summary for 1.6.2.4 extracted from
- The cytochrome P-450 alkane monooxygenase system of the yeast Lodderomyces elongisporus: purification and some properties of the NADPH-cytochrome P-450 reductase (1982), Biochem. Biophys. Res. Commun., 106, 1318-1324.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Nonionic detergent | activation | Lodderomyces elongisporus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Lodderomyces elongisporus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 79000 | - |
x * 79000, SDS-PAGE | Lodderomyces elongisporus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Lodderomyces elongisporus | acts as an electron transfer component of the alkane monoxygenase system | ? | - |
? |  |
| additional information | Lodderomyces elongisporus EH15D | acts as an electron transfer component of the alkane monoxygenase system | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lodderomyces elongisporus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-Sephacel and hydroxylapatite column chromatography | Lodderomyces elongisporus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 18.4 | - |
electron acceptor: 2,6-dichlorophenolindophenol | Lodderomyces elongisporus |
| 68.9 | - |
- |
Lodderomyces elongisporus |
| 98.3 | - |
electron acceptor: ferricyanide | Lodderomyces elongisporus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C | Lodderomyces elongisporus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 ferricytochrome c + NADPH | additional electron acceptor: ferricyanide | Lodderomyces elongisporus | 2 ferrocytochrome c + NADP+ + H+ | - |
? | |
| 2 ferricytochrome c + NADPH | additional electron acceptor: 2,6-dichlorophenolindophenol | Lodderomyces elongisporus | 2 ferrocytochrome c + NADP+ + H+ | - |
? | |
| 2 ferricytochrome c + NADPH | additional electron acceptor: ferricyanide | Lodderomyces elongisporus EH15D | 2 ferrocytochrome c + NADP+ + H+ | - |
? | |
| 2 ferricytochrome c + NADPH | additional electron acceptor: 2,6-dichlorophenolindophenol | Lodderomyces elongisporus EH15D | 2 ferrocytochrome c + NADP+ + H+ | - |
? | |
| additional information | acts as an electron transfer component of the alkane monoxygenase system | Lodderomyces elongisporus | ? | - |
? | |
| additional information | acts as an electron transfer component of the alkane monoxygenase system | Lodderomyces elongisporus EH15D | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 79000, SDS-PAGE | Lodderomyces elongisporus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | ratio FAD : FMN 1 : 1 | Lodderomyces elongisporus | |
| FMN | 1 mol per mol of enzyme | Lodderomyces elongisporus | |
| FMN | ratio FAD : FMN 1 : 1 | Lodderomyces elongisporus | |
| NADPH | - |
Lodderomyces elongisporus | |
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