Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Bos taurus | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ferricytochrome b5 + NADH | Bos taurus | - |
2 ferrocytochrome b5 + NAD+ + H+ | - |
? | |
additional information | Bos taurus | poor activity with the anticancer drug mitomycin C, no activity with anticancer drug idarubicin. The quinone antitumor agents are used in the treatment of several human neoplasms | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme from liver by anion-exchange chromatography of the detergent solubilized microsomes on two successive DEAE-cellulose columns and an affinity chromatography on adenosine 5'-diphosphate-agarose, to homogeneity | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ferricytochrome b5 + NADH | - |
Bos taurus | 2 ferrocytochrome b5 + NAD+ + H+ | - |
? | |
additional information | poor activity with the anticancer drug mitomycin C, no activity with anticancer drug idarubicin. The quinone antitumor agents are used in the treatment of several human neoplasms | Bos taurus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NADH-cytochrome b5 reductase | - |
Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.6 | 7.5 | assay at | Bos taurus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.6 | 7.5 | enzyme activity is higher at pH 7.5 compared to pH 6.6 | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Bos taurus |