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Literature summary for 1.6.2.2 extracted from

  • Deng, B.; Parthasarathy, S.; Wang, W.; Gibney, B.R.; Battaile, K.P.; Lovell, S.; Benson, D.R.; Zhu, H.
    Study of the individual cytochrome b5 and cytochrome b5 reductase domains of Ncb5or reveals a unique heme pocket and a possible role of the CS domain (2010), J. Biol. Chem., 285, 30181-30191.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.6.2.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 2 M (NH4)2SO4, 100 mM sodium/potassium phosphate, pH 6.2, 200 mM Li2SO4, at 4°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome Cyb5R3 Homo sapiens
-
-

Organism

Organism UniProt Comment Textmining
Homo sapiens Q7L1T6
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-NTA bead chromatography, HiTrap Q column chromatography, HiTrap phenyl column chromatography, and Superdex 200 gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ferricytochrome b5 + NADH
-
 Homo sapiens 2 ferrocytochrome b5 + NAD+ + H+
-
 ?

Synonyms

Synonyms Comment Organism
b5 plusb5R
-
 Homo sapiens
b5/b5R
-
 Homo sapiens
B5R cytochrome b5 reductase domain of Ncb5or Homo sapiens
CYB5R3
-
 Homo sapiens
Cyb5R4
-
 Homo sapiens
cytochrome b5 reductase
-
 Homo sapiens
NADH cytochrome b5 oxidoreductase
-
 Homo sapiens
Ncb5or
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Homo sapiens
NADH
-
 Homo sapiens