Protein Variants | Comment | Organism |
---|---|---|
G75S | natural mutation isolated in patient with recessive congenital methemoglobinaemia. Mutant retains stoichiometric levels of FAD, but shows decreased catalytic efficiency and reduced protein stability | Homo sapiens |
G75S/V252M | natural mutation isolated in patient with recessive congenital methemoglobinaemia. Mutant retains stoichiometric levels of FAD, but shows decreased catalytic efficiency and reduced protein stability | Homo sapiens |
V252M | natural mutation isolated in patient with recessive congenital methemoglobinaemia. Mutant retains stoichiometric levels of FAD, but shows decreased catalytic efficiency and reduced protein stability | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
NADH | wild-type | Homo sapiens | |
0.006 | - |
ferricyanide | wild-type | Homo sapiens | |
0.007 | - |
ferricyanide | mutant G75S | Homo sapiens | |
0.007 | - |
ferricyanide | mutant V252M | Homo sapiens | |
0.008 | - |
ferricyanide | mutant G75S/V252M | Homo sapiens | |
0.009 | - |
NADH | mutant G75S | Homo sapiens | |
0.013 | - |
ferricytochrome b5 | wild-type | Homo sapiens | |
0.013 | - |
ferricytochrome b5 | mutant V252M | Homo sapiens | |
0.014 | - |
ferricytochrome b5 | mutant G75S | Homo sapiens | |
0.014 | - |
ferricytochrome b5 | mutant G75S/V252M | Homo sapiens | |
0.017 | - |
NADH | mutant G75S/V252M | Homo sapiens | |
0.052 | - |
NADH | mutant V252M | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ferricyanide + NADH | - |
Homo sapiens | 2 ferrocyanide + NAD+ + H+ | - |
? | |
2 ferricytochrome b5 + NADH | - |
Homo sapiens | 2 ferrocytochrome b5 + NAD+ + H+ | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
60 | - |
ferricytochrome b5 | mutant G75S | Homo sapiens | |
85 | - |
ferricytochrome b5 | mutant G75S/V252M | Homo sapiens | |
133 | - |
ferricyanide | mutant G75S | Homo sapiens | |
187 | - |
ferricyanide | mutant G75S/V252M | Homo sapiens | |
285 | - |
ferricytochrome b5 | mutant V252M | Homo sapiens | |
360 | - |
ferricytochrome b5 | wild-type | Homo sapiens | |
633 | - |
ferricyanide | mutant V252M | Homo sapiens | |
800 | - |
ferricyanide | wild-type | Homo sapiens |